Establishment of cryopreserved gene banks of European chestnut and cork oak
Cryopreservation of selected genotypes of Europeanchestnut and cork oak was carried out in two laboratories in a proect involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (co...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/22592 |
| Acceso en línea: | http://hdl.handle.net/10261/22592 |
| Access Level: | acceso abierto |
| Palabra clave: | Castanea sativa Cryopreservation Forest tree species Liquid nitrogen Quercus suber Vitrification |
| Sumario: | Cryopreservation of selected genotypes of Europeanchestnut and cork oak was carried out in two laboratories in a proect involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty three out 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embrygenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to thse derived from non‐supercooled material. In light of the results obtained, long-term cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field |
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