Chimeras for the twenty-first century

Recent advances in stem cell biology and molecular engineering have improved and simplified the methodology employed to create experimental chimeras, highlighting their value in basic research and broadening the spectrum of potential applications. Experimental chimeras have been used for decades dur...

Descripción completa

Detalles Bibliográficos
Autores: Morata Tarifa, Cynthia, López Navas, Luis, Azkona Mendoza, Garikoitz, Sánchez Pernaute, Rosario
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/66515
Acceso en línea:http://hdl.handle.net/10810/66515
Access Level:acceso abierto
Palabra clave:blastocyst complementation
chimera
embryonic stem cells
ethics
interspecific
organogenesis
organoids
pluripotent stem cells
tissue engineering
Descripción
Sumario:Recent advances in stem cell biology and molecular engineering have improved and simplified the methodology employed to create experimental chimeras, highlighting their value in basic research and broadening the spectrum of potential applications. Experimental chimeras have been used for decades during the generation of murine genetic models, this being especially relevant in developmental and regeneration studies. Indeed, their value for the research and modeling of human diseases was recognized by the 2007 Nobel Prize to Mario Capecchi, Martin Evans, and Oliver Smithies. More recently, their potential application in regenerative medicine has generated a lot of interest, particularly the enticing possibility to generate human organs for transplantation in livestock animals. In this review, we provide an update on interspecific chimeric organogenesis, its possibilities, current limitations, alternatives, and ethical issues.