The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain

The release of 5-HT in terminal areas of the rodent brain is regulated by 5-HT1B receptors. Here we examined the role of 5-HT1B receptors in the control of 5-HT output and firing in the dorsal raphe nucleus (DR), median raphe nucleus (MnR) and forebrain of the rat in vivo. The local perfusion (30–30...

Descripción completa

Detalles Bibliográficos
Autores: Adell, Albert, Celada, Pau, Artigas, Francesc
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/33063
Acceso en línea:http://hdl.handle.net/10261/33063
Access Level:acceso abierto
Palabra clave:dorsal raphe nucleus
5-HT1B receptors
5-HT (5-hydroxytryptamine, serotonin) release
median raphe nucleus
single unit recording
id ES_065fa7a133bb8522dbcdf3fde9b9b781
oai_identifier_str oai:digital.csic.es:10261/33063
network_acronym_str ES
network_name_str España
repository_id_str
spelling The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brainAdell, AlbertCelada, PauArtigas, Francescdorsal raphe nucleus5-HT1B receptors5-HT (5-hydroxytryptamine, serotonin) releasemedian raphe nucleussingle unit recordingThe release of 5-HT in terminal areas of the rodent brain is regulated by 5-HT1B receptors. Here we examined the role of 5-HT1B receptors in the control of 5-HT output and firing in the dorsal raphe nucleus (DR), median raphe nucleus (MnR) and forebrain of the rat in vivo. The local perfusion (30–300 µm) of the selective 5-HT1B receptor agonist CP-93,129 to freely moving rats decreased 5-HT release in the DR and more markedly in the MnR. Likewise, 300 µm CP-93,129 reduced 5-HT output in substantia nigra pars reticulata, ventral pallidum, lateral habenula and the suprachiasmatic nucleus. The effect of CP-93,129 was prevented by SB-224289, but not by WAY-100635, selective 5-HT1B and 5-HT1A receptor antagonists, respectively. SB-224289 did not alter dialysate 5-HT in any raphe nuclei. The intravenous administration of the brain-penetrant selective 5-HT1B receptor agonist CP-94,253 (0.5–2.0 mg/kg) to anesthetized rats decreased dialysate 5-HT in dorsal hippocampus and globus pallidus, increased it in MnR and left it unaltered in the DR and medial prefrontal cortex. SB-224289, at a dose known to block 5-HT1B autoreceptor-mediated effects (5 mg/kg), did not prevent the effect of CP-94,253 on MnR 5-HT. The intravenous administration of CP-94,253 (0.05–1.6 mg/kg) to anesthetized rats increased the firing rate of MnR, but not DR-5-HT neurons. The local perfusion of CP-94,253 in the MnR showed a biphasic effect, with 5-HT reductions at 0.3–3 µm and increase at 300 µm. These results suggest that 5-HT cell firing and release in midbrain raphe nuclei (particularly in the MnR) are under control of 5-HT1B receptors. The activation of 5-HT1B autoreceptors (possibly located on 5-HT nerve endings and/or varicosities within DR and MnR) reduces 5-HT release. The effects of higher concentrations of 5-HT1B receptor agonists seem more compatible with the activation of 5-HT1B heteroreceptors on inhibitory neurons.Peer reviewedWiley-Blackwell201120112001info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/33063reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.2001.00550.x/abstracthttp://dx.doi.org/10.1046/j.1471-4159.2001.00550.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/330632026-05-22T06:33:51Z
dc.title.none.fl_str_mv The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
title The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
spellingShingle The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
Adell, Albert
dorsal raphe nucleus
5-HT1B receptors
5-HT (5-hydroxytryptamine, serotonin) release
median raphe nucleus
single unit recording
title_short The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
title_full The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
title_fullStr The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
title_full_unstemmed The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
title_sort The role of 5-HT1B receptors in the regulation of serotonin cell firing and release in the rat brain
dc.creator.none.fl_str_mv Adell, Albert
Celada, Pau
Artigas, Francesc
author Adell, Albert
author_facet Adell, Albert
Celada, Pau
Artigas, Francesc
author_role author
author2 Celada, Pau
Artigas, Francesc
author2_role author
author
dc.subject.none.fl_str_mv dorsal raphe nucleus
5-HT1B receptors
5-HT (5-hydroxytryptamine, serotonin) release
median raphe nucleus
single unit recording
topic dorsal raphe nucleus
5-HT1B receptors
5-HT (5-hydroxytryptamine, serotonin) release
median raphe nucleus
single unit recording
description The release of 5-HT in terminal areas of the rodent brain is regulated by 5-HT1B receptors. Here we examined the role of 5-HT1B receptors in the control of 5-HT output and firing in the dorsal raphe nucleus (DR), median raphe nucleus (MnR) and forebrain of the rat in vivo. The local perfusion (30–300 µm) of the selective 5-HT1B receptor agonist CP-93,129 to freely moving rats decreased 5-HT release in the DR and more markedly in the MnR. Likewise, 300 µm CP-93,129 reduced 5-HT output in substantia nigra pars reticulata, ventral pallidum, lateral habenula and the suprachiasmatic nucleus. The effect of CP-93,129 was prevented by SB-224289, but not by WAY-100635, selective 5-HT1B and 5-HT1A receptor antagonists, respectively. SB-224289 did not alter dialysate 5-HT in any raphe nuclei. The intravenous administration of the brain-penetrant selective 5-HT1B receptor agonist CP-94,253 (0.5–2.0 mg/kg) to anesthetized rats decreased dialysate 5-HT in dorsal hippocampus and globus pallidus, increased it in MnR and left it unaltered in the DR and medial prefrontal cortex. SB-224289, at a dose known to block 5-HT1B autoreceptor-mediated effects (5 mg/kg), did not prevent the effect of CP-94,253 on MnR 5-HT. The intravenous administration of CP-94,253 (0.05–1.6 mg/kg) to anesthetized rats increased the firing rate of MnR, but not DR-5-HT neurons. The local perfusion of CP-94,253 in the MnR showed a biphasic effect, with 5-HT reductions at 0.3–3 µm and increase at 300 µm. These results suggest that 5-HT cell firing and release in midbrain raphe nuclei (particularly in the MnR) are under control of 5-HT1B receptors. The activation of 5-HT1B autoreceptors (possibly located on 5-HT nerve endings and/or varicosities within DR and MnR) reduces 5-HT release. The effects of higher concentrations of 5-HT1B receptor agonists seem more compatible with the activation of 5-HT1B heteroreceptors on inhibitory neurons.
publishDate 2001
dc.date.none.fl_str_mv 2001
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/33063
url http://hdl.handle.net/10261/33063
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.2001.00550.x/abstract
http://dx.doi.org/10.1046/j.1471-4159.2001.00550.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402914046345216
score 15,811543

Similares en LA Referencia