SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta

The aim is to quantitatively analyze the diameter and tubular density and semi-quantitatively analyze the elemental composition (Calcium and Phosphorus) in the dentin of primary teeth of children with Osteogenesis Imperfecta (OI) in comparison with a control group. Material and methods: A microstruc...

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Autores: Martín Vacas, Andrea, Vera González, Vicente, Ramírez Castellanos, Julio, González Gil, Diego, Nova García, Manuel Joaquín De
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102324
Acesso em linha:https://hdl.handle.net/20.500.14352/102324
Access Level:acceso abierto
Palavra-chave:616.314-053.2
616.71
615.849
Osteogenesis imperfecta
Dentistry
Rare diseases
Dentinogenesis imperfecta
Primary teeth
Pediatric dentistry
Ciencias Biomédicas
Odontología infantil
Diagnóstico por imagen y medicina nuclear
32 Ciencias Médicas
3207.14 Osteopatología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/102324
network_acronym_str ES
network_name_str España
repository_id_str
spelling SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfectaMartín Vacas, AndreaVera González, VicenteRamírez Castellanos, JulioGonzález Gil, DiegoNova García, Manuel Joaquín De616.314-053.2616.71615.849Osteogenesis imperfectaDentistryRare diseasesDentinogenesis imperfectaPrimary teethPediatric dentistryCiencias BiomédicasOdontología infantilDiagnóstico por imagen y medicina nuclear32 Ciencias Médicas3207.14 OsteopatologíaThe aim is to quantitatively analyze the diameter and tubular density and semi-quantitatively analyze the elemental composition (Calcium and Phosphorus) in the dentin of primary teeth of children with Osteogenesis Imperfecta (OI) in comparison with a control group. Material and methods: A microstructural (in vitro) analysis of primary teeth of children with OI was performed with SEM and EDS. The variables measured were the tubule count (tubule/mm2) and diameter (µm) at 2000 times magnification at four points of the dentin of different depths. A semiquantitative analysis of the elemental composition of the dentin was performed with EDS of Calcium and Phosphorus (cps). Descriptive and inferential analysis (Fisher’s exact test, the Mann–Whitney U test, the Kruskal–Wallis test, the Bonferroni post hoc test, the ANOVA test of repeated measures, a test of inter- and intra-subject effects, and the Geisser–Greenhouse test) were carried out. Data were analyzed with a 95% confidence level (p-value < 0.05). Results: A total of 25 deciduous teeth from 17 patients with OI and 30 teeth from healthy children were studied. There were differences in the count and tubular diameter for the control group with OI; in addition, the behavior curve changed when the systemic disease was severe. While there were no differences in the amount of dentinal Calcium, the OI tooth showed a significantly lower amount of Phosphorus (p < 0.05), except in the pulpal dentin (p > 0.05). Conclusion: The alterations of the dentinal tubules (density and diameter) were more pronounced in the most severe phenotypes of systemic disease. The amount of Phosphorus was decreased in the dentin of the primary teeth of children with OI compared to the control group.MDPIUniversidad Complutense de Madrid20232023-11-1720232023-11-17journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102324reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1023242026-06-02T12:44:21Z
dc.title.none.fl_str_mv SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
title SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
spellingShingle SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
Martín Vacas, Andrea
616.314-053.2
616.71
615.849
Osteogenesis imperfecta
Dentistry
Rare diseases
Dentinogenesis imperfecta
Primary teeth
Pediatric dentistry
Ciencias Biomédicas
Odontología infantil
Diagnóstico por imagen y medicina nuclear
32 Ciencias Médicas
3207.14 Osteopatología
title_short SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
title_full SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
title_fullStr SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
title_full_unstemmed SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
title_sort SEM/EDS analysis of tubules count and mineral deposition in the dentin of children with osteogenesis imperfecta
dc.creator.none.fl_str_mv Martín Vacas, Andrea
Vera González, Vicente
Ramírez Castellanos, Julio
González Gil, Diego
Nova García, Manuel Joaquín De
author Martín Vacas, Andrea
author_facet Martín Vacas, Andrea
Vera González, Vicente
Ramírez Castellanos, Julio
González Gil, Diego
Nova García, Manuel Joaquín De
author_role author
author2 Vera González, Vicente
Ramírez Castellanos, Julio
González Gil, Diego
Nova García, Manuel Joaquín De
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616.314-053.2
616.71
615.849
Osteogenesis imperfecta
Dentistry
Rare diseases
Dentinogenesis imperfecta
Primary teeth
Pediatric dentistry
Ciencias Biomédicas
Odontología infantil
Diagnóstico por imagen y medicina nuclear
32 Ciencias Médicas
3207.14 Osteopatología
topic 616.314-053.2
616.71
615.849
Osteogenesis imperfecta
Dentistry
Rare diseases
Dentinogenesis imperfecta
Primary teeth
Pediatric dentistry
Ciencias Biomédicas
Odontología infantil
Diagnóstico por imagen y medicina nuclear
32 Ciencias Médicas
3207.14 Osteopatología
description The aim is to quantitatively analyze the diameter and tubular density and semi-quantitatively analyze the elemental composition (Calcium and Phosphorus) in the dentin of primary teeth of children with Osteogenesis Imperfecta (OI) in comparison with a control group. Material and methods: A microstructural (in vitro) analysis of primary teeth of children with OI was performed with SEM and EDS. The variables measured were the tubule count (tubule/mm2) and diameter (µm) at 2000 times magnification at four points of the dentin of different depths. A semiquantitative analysis of the elemental composition of the dentin was performed with EDS of Calcium and Phosphorus (cps). Descriptive and inferential analysis (Fisher’s exact test, the Mann–Whitney U test, the Kruskal–Wallis test, the Bonferroni post hoc test, the ANOVA test of repeated measures, a test of inter- and intra-subject effects, and the Geisser–Greenhouse test) were carried out. Data were analyzed with a 95% confidence level (p-value < 0.05). Results: A total of 25 deciduous teeth from 17 patients with OI and 30 teeth from healthy children were studied. There were differences in the count and tubular diameter for the control group with OI; in addition, the behavior curve changed when the systemic disease was severe. While there were no differences in the amount of dentinal Calcium, the OI tooth showed a significantly lower amount of Phosphorus (p < 0.05), except in the pulpal dentin (p > 0.05). Conclusion: The alterations of the dentinal tubules (density and diameter) were more pronounced in the most severe phenotypes of systemic disease. The amount of Phosphorus was decreased in the dentin of the primary teeth of children with OI compared to the control group.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-11-17
2023
2023-11-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102324
url https://hdl.handle.net/20.500.14352/102324
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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