Antibacterial hybrid nanoparticles coating for inhibition of bacterial growth and biofilm formation
Contact lenses (CLs) are medical devices widely used for vision correction, cosmetic or therapeutic reasons. CLs are ideal surface for bacteria to adhere and establish antibiotic resistant biofilms, being the Pseudomonas aeruginosa (P. aeruginosa) the most frequently isolated bacterial pathogen caus...
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| Tipo de documento: | dissertação |
| Data de publicação: | 2020 |
| País: | España |
| Recursos: | Universitat Politècnica de Catalunya (UPC) |
| Repositório: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglês |
| OAI Identifier: | oai:upcommons.upc.edu:2117/336770 |
| Acesso em linha: | https://hdl.handle.net/2117/336770 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Contact lenses Nanoparticles Lents de contacte Lents de contacte -- Microbiologia Nanopartícules Àrees temàtiques de la UPC::Ciències de la visió::Contactologia |
| Resumo: | Contact lenses (CLs) are medical devices widely used for vision correction, cosmetic or therapeutic reasons. CLs are ideal surface for bacteria to adhere and establish antibiotic resistant biofilms, being the Pseudomonas aeruginosa (P. aeruginosa) the most frequently isolated bacterial pathogen causing CLs-associated microbial keratitis. Their use also introduces numerous factors (e.g. patient compliance and hand hygiene) that increase the ability of bacteria to interact with the cornea and cause difficult to treat infections. In the present study, a hybrid nanocomposite coating of antimicrobial silver-Cruox nanoparticles (AgCru NPs), anti-fouling poly(sulfobetaine methacrylate) (pSBMA) and biofilm-matrix degrading enzyme a-Amylase has been developed onto silicone hydrogel CLs in a one-step sonochemical process to impart antibacterial and antibiofilm functionalities. Hybrid AgCru NPs with antibacterial activity against P. aeruginosa have been produced in a green synthetic approach using Cruox - a bio-liquefied wheat bran containing ferulic acid, di-ferulic acids and arabinoxylans - as both reducing and capping agent. Coating the CLs with hybrid NPs under high intensity ultrasound did not alter their refractive properties and water content. Moreover, the CLs´ functionalization led to improved wettability compared to the pristine CLs and demonstrated strong capacity to reduce the growth and biofilm formation of P. aeruginosa by 6.4 and 1.7 Log (CFU/mL), respectively. The novel nanocomposite coatings were biocompatible to human cells and as a such are of great potential to prevent CLs-wearing associated bacterial infections |
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