Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1

The production, biochemical characterization, and carbohydrate specificity of LacA β-galactosidase (locus lp_3469) belonging to the glycoside hydrolase family 42 from the probiotic organism Lactobacillus plantarum WCFS1 are addressed. The β-d-galactosidase activity was maximal in the pH range of 4.0...

ver descrição completa

Detalhes bibliográficos
Autores: Delgado, Paloma, Plaza-Vinuesa, Laura, Lizasoain, Silvia, De Las Rivas, Blanca, Muñoz, Rosario, Jimeno, M. Luisa, Doyagüez, Elisa G., Moreno, F. Javier, Corzo, Nieves
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/226779
Acesso em linha:http://hdl.handle.net/10261/226779
Access Level:acceso abierto
Palavra-chave:β-galactosidase
Lactobacillus plantarum WCFS1
Prebiotics
Polyalcohols
Polyols
id ES_01d52f6e93afcd78b3ccd4d2e998bf42
oai_identifier_str oai:digital.csic.es:10261/226779
network_acronym_str ES
network_name_str España
repository_id_str
spelling Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1Delgado, PalomaPlaza-Vinuesa, LauraLizasoain, SilviaDe Las Rivas, BlancaMuñoz, RosarioJimeno, M. LuisaDoyagüez, Elisa G.Moreno, F. JavierCorzo, Nievesβ-galactosidaseLactobacillus plantarum WCFS1PrebioticsPolyalcoholsPolyolsThe production, biochemical characterization, and carbohydrate specificity of LacA β-galactosidase (locus lp_3469) belonging to the glycoside hydrolase family 42 from the probiotic organism Lactobacillus plantarum WCFS1 are addressed. The β-d-galactosidase activity was maximal in the pH range of 4.0-7.0 and at 30-37 °C. High hydrolysis capacity toward the β(1 → 4) linkages between galactose and glucose (lactose) or fructose (lactulose) was found. High efficiency toward galactosyl derivative formation was observed when lactose and glycerol, xylitol, or erythritol were used. Galactosyl derivatives of xylitol were characterized for the first time as 3-O-β-d-galactopyranosyl-xylitol and 1-O-β-d-galactopyranosyl-xylitol, displaying high preference of LacA β-galactosidase for the transfer of galactosyl residues from lactose to the C1 or C3 hydroxyl group of xylitol. These results indicate the feasibility of using LacA β-galactosidase for the synthesis of different galactosyl-polyols, which could be promising candidates for beneficial and appealing functional and technological applications such as novel prebiotics or hypocaloric sweeteners.This work has been financed by the Spanish Ministry of Economy, Industry and Competitiveness (Projects AGL2017- 84614-C2-1-R and AGL2017-84614-C2-2-R).American Chemical SocietyMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/226779reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-2-RAGL2017-84614-C2-1-R/AEI/10.13039/501100011033AGL2017-84614-C2-2-R/AEI/10.13039/501100011033http://dx.doi.org/10.1021/acs.jafc.0c02439Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2267792026-05-22T06:33:51Z
dc.title.none.fl_str_mv Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
title Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
spellingShingle Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
Delgado, Paloma
β-galactosidase
Lactobacillus plantarum WCFS1
Prebiotics
Polyalcohols
Polyols
title_short Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_full Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_fullStr Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_full_unstemmed Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
title_sort Hydrolysis of lactose and transglycosylation of selected sugar alcohols by LacA β-galactosidase from Lactobacillus plantarum WCFS1
dc.creator.none.fl_str_mv Delgado, Paloma
Plaza-Vinuesa, Laura
Lizasoain, Silvia
De Las Rivas, Blanca
Muñoz, Rosario
Jimeno, M. Luisa
Doyagüez, Elisa G.
Moreno, F. Javier
Corzo, Nieves
author Delgado, Paloma
author_facet Delgado, Paloma
Plaza-Vinuesa, Laura
Lizasoain, Silvia
De Las Rivas, Blanca
Muñoz, Rosario
Jimeno, M. Luisa
Doyagüez, Elisa G.
Moreno, F. Javier
Corzo, Nieves
author_role author
author2 Plaza-Vinuesa, Laura
Lizasoain, Silvia
De Las Rivas, Blanca
Muñoz, Rosario
Jimeno, M. Luisa
Doyagüez, Elisa G.
Moreno, F. Javier
Corzo, Nieves
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv β-galactosidase
Lactobacillus plantarum WCFS1
Prebiotics
Polyalcohols
Polyols
topic β-galactosidase
Lactobacillus plantarum WCFS1
Prebiotics
Polyalcohols
Polyols
description The production, biochemical characterization, and carbohydrate specificity of LacA β-galactosidase (locus lp_3469) belonging to the glycoside hydrolase family 42 from the probiotic organism Lactobacillus plantarum WCFS1 are addressed. The β-d-galactosidase activity was maximal in the pH range of 4.0-7.0 and at 30-37 °C. High hydrolysis capacity toward the β(1 → 4) linkages between galactose and glucose (lactose) or fructose (lactulose) was found. High efficiency toward galactosyl derivative formation was observed when lactose and glycerol, xylitol, or erythritol were used. Galactosyl derivatives of xylitol were characterized for the first time as 3-O-β-d-galactopyranosyl-xylitol and 1-O-β-d-galactopyranosyl-xylitol, displaying high preference of LacA β-galactosidase for the transfer of galactosyl residues from lactose to the C1 or C3 hydroxyl group of xylitol. These results indicate the feasibility of using LacA β-galactosidase for the synthesis of different galactosyl-polyols, which could be promising candidates for beneficial and appealing functional and technological applications such as novel prebiotics or hypocaloric sweeteners.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/226779
url http://hdl.handle.net/10261/226779
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84614-C2-2-R
AGL2017-84614-C2-1-R/AEI/10.13039/501100011033
AGL2017-84614-C2-2-R/AEI/10.13039/501100011033
http://dx.doi.org/10.1021/acs.jafc.0c02439
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402612390952960
score 15.812429

Registos relacionados