Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells

Functionalized fluorescent silica nanoparticles were designed and synthesized to selectively target cancer cells for bioimaging analysis. The synthesis method and characterization of functionalized fluorescent silica nanoparticles (50–60 nm), as well as internalization and subcellular localization i...

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Autores: Prieto Montero, Ruth, Katsumiti Kodo Filho, Alberto, Cajaraville Bereciartua, Miren Pilare, López Arbeloa, Iñigo María, Martínez Martínez, Virginia
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/46915
Acceso en línea:http://hdl.handle.net/10810/46915
Access Level:acceso abierto
Palabra clave:targeting
functionalized fluorescent silica nanoparticles
rhodamine 101
polyethylene glycol
folic acid
HeLa cells
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spelling Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer CellsPrieto Montero, RuthKatsumiti Kodo Filho, AlbertoCajaraville Bereciartua, Miren PilareLópez Arbeloa, Iñigo MaríaMartínez Martínez, Virginiatargetingfunctionalized fluorescent silica nanoparticlesrhodamine 101polyethylene glycolfolic acidHeLa cellsFunctionalized fluorescent silica nanoparticles were designed and synthesized to selectively target cancer cells for bioimaging analysis. The synthesis method and characterization of functionalized fluorescent silica nanoparticles (50–60 nm), as well as internalization and subcellular localization in HeLa cells is reported here. The dye, rhodamine 101 (R101) was physically embedded during the sol–gel synthesis. The dye loading was optimized by varying the synthesis conditions (temperature and dye concentration added to the gel) and by the use of different organotriethoxysilanes as a second silica precursor. Additionally, R101, was also covalently bound to the functionalized external surface of the silica nanoparticles. The quantum yields of the dye-doped silica nanoparticles range from 0.25 to 0.50 and demonstrated an enhanced brightness of 230–260 fold respect to the free dye in solution. The shell of the nanoparticles was further decorated with PEG of 2000 Da and folic acid (FA) to ensure good stability in water and to enhance selectivity to cancer cells, respectively. In vitro assays with HeLa cells showed that fluorescent nanoparticles were internalized by cells accumulating exclusively into lysosomes. Quantitative analysis showed a significantly higher accumulation of FA functionalized fluorescent silica nanoparticles compared to nanoparticles without FA, proving that the former may represent good candidates for targeting cancer cells.This research was funded by the Basque Government, grant numbers IT912-16 and IT-1302-19; Ministry of Economy and Competitiveness (MINECO), grant numbers MAT2017-83856-C3-3-P and CTM2016-81130-R; and the University of the Basque Country (UPV/EHU), grant number COLAB19/01.MDPI2020202020202020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/46915reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/MAT2017-83856-C3-3-P/info:eu-repo/grantAgreement/MINECO/CTM2016-81130-R/https://www.mdpi.com/1424-8220/20/19/5590info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/469152026-06-18T09:23:17Z
dc.title.none.fl_str_mv Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
title Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
spellingShingle Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
Prieto Montero, Ruth
targeting
functionalized fluorescent silica nanoparticles
rhodamine 101
polyethylene glycol
folic acid
HeLa cells
title_short Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
title_full Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
title_fullStr Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
title_full_unstemmed Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
title_sort Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells
dc.creator.none.fl_str_mv Prieto Montero, Ruth
Katsumiti Kodo Filho, Alberto
Cajaraville Bereciartua, Miren Pilare
López Arbeloa, Iñigo María
Martínez Martínez, Virginia
author Prieto Montero, Ruth
author_facet Prieto Montero, Ruth
Katsumiti Kodo Filho, Alberto
Cajaraville Bereciartua, Miren Pilare
López Arbeloa, Iñigo María
Martínez Martínez, Virginia
author_role author
author2 Katsumiti Kodo Filho, Alberto
Cajaraville Bereciartua, Miren Pilare
López Arbeloa, Iñigo María
Martínez Martínez, Virginia
author2_role author
author
author
author
dc.subject.none.fl_str_mv targeting
functionalized fluorescent silica nanoparticles
rhodamine 101
polyethylene glycol
folic acid
HeLa cells
topic targeting
functionalized fluorescent silica nanoparticles
rhodamine 101
polyethylene glycol
folic acid
HeLa cells
description Functionalized fluorescent silica nanoparticles were designed and synthesized to selectively target cancer cells for bioimaging analysis. The synthesis method and characterization of functionalized fluorescent silica nanoparticles (50–60 nm), as well as internalization and subcellular localization in HeLa cells is reported here. The dye, rhodamine 101 (R101) was physically embedded during the sol–gel synthesis. The dye loading was optimized by varying the synthesis conditions (temperature and dye concentration added to the gel) and by the use of different organotriethoxysilanes as a second silica precursor. Additionally, R101, was also covalently bound to the functionalized external surface of the silica nanoparticles. The quantum yields of the dye-doped silica nanoparticles range from 0.25 to 0.50 and demonstrated an enhanced brightness of 230–260 fold respect to the free dye in solution. The shell of the nanoparticles was further decorated with PEG of 2000 Da and folic acid (FA) to ensure good stability in water and to enhance selectivity to cancer cells, respectively. In vitro assays with HeLa cells showed that fluorescent nanoparticles were internalized by cells accumulating exclusively into lysosomes. Quantitative analysis showed a significantly higher accumulation of FA functionalized fluorescent silica nanoparticles compared to nanoparticles without FA, proving that the former may represent good candidates for targeting cancer cells.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/46915
url http://hdl.handle.net/10810/46915
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/MAT2017-83856-C3-3-P/
info:eu-repo/grantAgreement/MINECO/CTM2016-81130-R/
https://www.mdpi.com/1424-8220/20/19/5590
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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