Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans

8 páginas, 8 figuras, 2 tablas -- PAGS nros.6391-6398

Detalles Bibliográficos
Autores: Rodríguez-Colinas, Bárbara, Poveda, Ana, Jiménez-Barbero, Jesús, Ballesteros Olmo, Antonio, Plou Gasca, Francisco José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/60340
Acceso en línea:http://hdl.handle.net/10261/60340
Access Level:acceso abierto
Palabra clave:Glycosidase
galacto-oligosaccharides
Prebiotics
transglycosylation
β-galactosidase
oligosaccharides
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spelling Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulansRodríguez-Colinas, BárbaraPoveda, AnaJiménez-Barbero, JesúsBallesteros Olmo, AntonioPlou Gasca, Francisco JoséGlycosidasegalacto-oligosaccharidesPrebioticstransglycosylationβ-galactosidaseoligosaccharides8 páginas, 8 figuras, 2 tablas -- PAGS nros.6391-6398The synthesis of galacto-oligosaccharides (GOS) catalyzed by a novel commercial preparation of β-galactosidase from Bacillus circulans (Biolactase) was studied, and the products were characterized by MS and NMR. Using 400 g/L lactose and 1.5 enzyme units per milliliter, the maximum GOS yield, measured by HPAEC-PAD analysis, was 165 g/L (41% w/w of total carbohydrates in the mixture). The major transgalactosylation products were the trisaccharide Gal-β(1→4)-Gal-β(1→4)-Glc and the tetrasaccharide Gal-β(1→4)-Gal-β(1→4)-Gal-β(1→4)-Glc. The GOS yield increased to 198 g/L (49.4% w/w of total carbohydrates) using a higher enzyme concentration (15 U/mL), which minimized the enzyme inactivation under reaction conditions. Using skim milk (with a lactose concentration of 46 g/L), the enzyme also displayed transgalactosylation activity: maximum GOS yield accounted for 15.4% (7.1 g/L), which was obtained at 50% lactose conversionProject BIO2010-20508-C04-01 from the Spanish Ministry of Science and Innovation supported this research. B.R.-C. was supported by a fellowship from the Spanish Ministry of Science and Innovation (FPI program)Peer reviewedAmerican Chemical Society201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/60340reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1021/jf301156vinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/603402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
title Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
spellingShingle Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
Rodríguez-Colinas, Bárbara
Glycosidase
galacto-oligosaccharides
Prebiotics
transglycosylation
β-galactosidase
oligosaccharides
title_short Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
title_full Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
title_fullStr Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
title_full_unstemmed Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
title_sort Galacto-oligosaccharide Synthesis from Lactose Solution or Skim Milk Using the β-Galactosidase from Bacillus circulans
dc.creator.none.fl_str_mv Rodríguez-Colinas, Bárbara
Poveda, Ana
Jiménez-Barbero, Jesús
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
author Rodríguez-Colinas, Bárbara
author_facet Rodríguez-Colinas, Bárbara
Poveda, Ana
Jiménez-Barbero, Jesús
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
author_role author
author2 Poveda, Ana
Jiménez-Barbero, Jesús
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Glycosidase
galacto-oligosaccharides
Prebiotics
transglycosylation
β-galactosidase
oligosaccharides
topic Glycosidase
galacto-oligosaccharides
Prebiotics
transglycosylation
β-galactosidase
oligosaccharides
description 8 páginas, 8 figuras, 2 tablas -- PAGS nros.6391-6398
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/60340
url http://hdl.handle.net/10261/60340
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1021/jf301156v
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402534273089536
score 15.81155

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