Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release

This paper discusses the development and validation of a rapid method for the reversed phase HPLC-UV quantification of biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) micro-spheres co-loaded with two neuroprotective agents (dexamethasone and melatonin) (DX-MEL-MSs) to be intravitreally admini...

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Authors: Vicario De La Torre, Marta, Herrero Vanrell, María Del Rocío, Andrés Guerrero, Vanesa, Bravo Osuna, Irene, Molina Martínez, Irene Teresa, Brugnera, Marco
Format: article
Publication Date:2022
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/115544
Online Access:https://hdl.handle.net/20.500.14352/115544
Access Level:Open access
Keyword:615.4
617.7
Glaucoma
Melatonin
Dexamethasone
Validation
HPLC-UV
PLGA
Microspheres
Co-delivery
Encapsulation efficiency
In vitro release
Tecnología farmaceútica
Oftalmología
3201.09 Oftalmología
2403 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/115544
network_acronym_str ES
network_name_str España
repository_id_str
spelling Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro releaseVicario De La Torre, MartaHerrero Vanrell, María Del RocíoAndrés Guerrero, VanesaBravo Osuna, IreneMolina Martínez, Irene TeresaBrugnera, Marco615.4617.7GlaucomaMelatoninDexamethasoneValidationHPLC-UVPLGAMicrospheresCo-deliveryEncapsulation efficiencyIn vitro releaseTecnología farmaceúticaOftalmología3201.09 Oftalmología2403 BioquímicaThis paper discusses the development and validation of a rapid method for the reversed phase HPLC-UV quantification of biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) micro-spheres co-loaded with two neuroprotective agents (dexamethasone and melatonin) (DX-MEL-MSs) to be intravitreally administered as a promising glaucoma treatment. The study was performed to validate two procedures that quantify the content of the two active substances entrapped into the polymer matrix during an encapsulation efficiency assay and the amount of drugs liberated over time during the in vitro release assay. The reversed-phase method allowed for the simultaneous determination of dexamethasone and melatonin, which were respectively detected at 240.5 and 222.7 nm. Chromatographic separation was performed using an Ascentis® C18 HPLC Column (25 cm × 4.6 mm, 5 μm) with an isocratic mobile phase composed of methanol-water (70:30, v/v) with 1.0 mL min−1 flow rate. The two procedures were validated analytically in terms of system suitability testing, specificity, linearity, precision, accuracy, sensitivity, and robustness. Both the validated procedures were applied to characterize DX-MEL-MSs and were found appropriate to quantify the drug quantities encapsulated and estimate their release profile over 10 days. The validation study designed in this work can be helpful for planning any other protocols that refer to the quantification of PLGA based drug delivery systems.MDPIUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/115544reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1155442026-06-02T12:44:21Z
dc.title.none.fl_str_mv Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
title Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
spellingShingle Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
Vicario De La Torre, Marta
615.4
617.7
Glaucoma
Melatonin
Dexamethasone
Validation
HPLC-UV
PLGA
Microspheres
Co-delivery
Encapsulation efficiency
In vitro release
Tecnología farmaceútica
Oftalmología
3201.09 Oftalmología
2403 Bioquímica
title_short Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
title_full Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
title_fullStr Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
title_full_unstemmed Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
title_sort Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
dc.creator.none.fl_str_mv Vicario De La Torre, Marta
Herrero Vanrell, María Del Rocío
Andrés Guerrero, Vanesa
Bravo Osuna, Irene
Molina Martínez, Irene Teresa
Brugnera, Marco
author Vicario De La Torre, Marta
author_facet Vicario De La Torre, Marta
Herrero Vanrell, María Del Rocío
Andrés Guerrero, Vanesa
Bravo Osuna, Irene
Molina Martínez, Irene Teresa
Brugnera, Marco
author_role author
author2 Herrero Vanrell, María Del Rocío
Andrés Guerrero, Vanesa
Bravo Osuna, Irene
Molina Martínez, Irene Teresa
Brugnera, Marco
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.4
617.7
Glaucoma
Melatonin
Dexamethasone
Validation
HPLC-UV
PLGA
Microspheres
Co-delivery
Encapsulation efficiency
In vitro release
Tecnología farmaceútica
Oftalmología
3201.09 Oftalmología
2403 Bioquímica
topic 615.4
617.7
Glaucoma
Melatonin
Dexamethasone
Validation
HPLC-UV
PLGA
Microspheres
Co-delivery
Encapsulation efficiency
In vitro release
Tecnología farmaceútica
Oftalmología
3201.09 Oftalmología
2403 Bioquímica
description This paper discusses the development and validation of a rapid method for the reversed phase HPLC-UV quantification of biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) micro-spheres co-loaded with two neuroprotective agents (dexamethasone and melatonin) (DX-MEL-MSs) to be intravitreally administered as a promising glaucoma treatment. The study was performed to validate two procedures that quantify the content of the two active substances entrapped into the polymer matrix during an encapsulation efficiency assay and the amount of drugs liberated over time during the in vitro release assay. The reversed-phase method allowed for the simultaneous determination of dexamethasone and melatonin, which were respectively detected at 240.5 and 222.7 nm. Chromatographic separation was performed using an Ascentis® C18 HPLC Column (25 cm × 4.6 mm, 5 μm) with an isocratic mobile phase composed of methanol-water (70:30, v/v) with 1.0 mL min−1 flow rate. The two procedures were validated analytically in terms of system suitability testing, specificity, linearity, precision, accuracy, sensitivity, and robustness. Both the validated procedures were applied to characterize DX-MEL-MSs and were found appropriate to quantify the drug quantities encapsulated and estimate their release profile over 10 days. The validation study designed in this work can be helpful for planning any other protocols that refer to the quantification of PLGA based drug delivery systems.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/115544
url https://hdl.handle.net/20.500.14352/115544
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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