Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery

Intranasal drug delivery to the brain offers a promising strategy to overcome biological barriers. Chitosan-coated nanoemulsion-based nanocapsules demonstrate significant potential due to their mucoadhesive properties, ability to permeate epithelial cells, and ability to solubilize poorly water-solu...

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Autores: Alleva, María, Baranyai, Zsuzsa, Esteban-Pérez, Natalia, Martínez-Vicente, Pablo, Martín-Rapún, Rafael, Moros, María, Martínez de la Fuente, Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:156599
Acceso en línea:http://zaguan.unizar.es/record/156599
Access Level:acceso abierto
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spelling Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain deliveryAlleva, MaríaBaranyai, ZsuzsaEsteban-Pérez, NataliaMartínez-Vicente, PabloMartín-Rapún, RafaelMoros, MaríaMartínez de la Fuente, JesúsIntranasal drug delivery to the brain offers a promising strategy to overcome biological barriers. Chitosan-coated nanoemulsion-based nanocapsules demonstrate significant potential due to their mucoadhesive properties, ability to permeate epithelial cells, and ability to solubilize poorly water-soluble drugs, making them ideal candidates for bypassing the blood-brain barrier and overcoming the nasal mucosa. To ensure effective drug delivery, it is critical to assess the integrity of these nanocapsules during their transit across such barriers. In this study, we employed Förster resonance energy transfer to track the structural integrity of nanocapsules during transport. A simplified in vitro model was established using Calu-3 cells to mimic the mucosal epithelial barrier and Balb-c 3T3 fibroblasts as target cells. Our findings demonstrated that the nanoemulsion core of the nanocapsules successfully crossed the in vitro epithelial barrier and reached target cells while maintaining its structural integrity. These results validate the potential of chitosan-coated nanocapsules as a robust platform for the intranasal delivery of drugs to the brain.2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/156599reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/DGA/E15-20Rinfo:eu-repo/grantAgreement/EC/H2020/842652 This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 842652 -TBNANOinfo:eu-repo/grantAgreement/ES/ISCIII/CB16-01info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-Sinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:1565992026-05-29T13:59:51Z
dc.title.none.fl_str_mv Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
title Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
spellingShingle Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
Alleva, María
title_short Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
title_full Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
title_fullStr Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
title_full_unstemmed Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
title_sort Förster resonance energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
dc.creator.none.fl_str_mv Alleva, María
Baranyai, Zsuzsa
Esteban-Pérez, Natalia
Martínez-Vicente, Pablo
Martín-Rapún, Rafael
Moros, María
Martínez de la Fuente, Jesús
author Alleva, María
author_facet Alleva, María
Baranyai, Zsuzsa
Esteban-Pérez, Natalia
Martínez-Vicente, Pablo
Martín-Rapún, Rafael
Moros, María
Martínez de la Fuente, Jesús
author_role author
author2 Baranyai, Zsuzsa
Esteban-Pérez, Natalia
Martínez-Vicente, Pablo
Martín-Rapún, Rafael
Moros, María
Martínez de la Fuente, Jesús
author2_role author
author
author
author
author
author
description Intranasal drug delivery to the brain offers a promising strategy to overcome biological barriers. Chitosan-coated nanoemulsion-based nanocapsules demonstrate significant potential due to their mucoadhesive properties, ability to permeate epithelial cells, and ability to solubilize poorly water-soluble drugs, making them ideal candidates for bypassing the blood-brain barrier and overcoming the nasal mucosa. To ensure effective drug delivery, it is critical to assess the integrity of these nanocapsules during their transit across such barriers. In this study, we employed Förster resonance energy transfer to track the structural integrity of nanocapsules during transport. A simplified in vitro model was established using Calu-3 cells to mimic the mucosal epithelial barrier and Balb-c 3T3 fibroblasts as target cells. Our findings demonstrated that the nanoemulsion core of the nanocapsules successfully crossed the in vitro epithelial barrier and reached target cells while maintaining its structural integrity. These results validate the potential of chitosan-coated nanocapsules as a robust platform for the intranasal delivery of drugs to the brain.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/156599
url http://zaguan.unizar.es/record/156599
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/DGA/E15-20R
info:eu-repo/grantAgreement/EC/H2020/842652
This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 842652 -TBNANO
info:eu-repo/grantAgreement/ES/ISCIII/CB16-01
info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00
info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-S
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
repository.name.fl_str_mv
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