Supporting Information: Förster Resonance Energy Transfer (FRET) demonstrates in vitro chitosan-coated nanocapsules suitability for intranasal brain delivery
General procedures; establishment and characterization of a simplified in vitro nasal mucosal barrier model using Calu-3 cells; simplified in vitro nasal mucosal barrier model with Calu-3; morphological, histological, and histochemical structure study of the Calu-3 model barrier; cell junction prote...
| Autores: | , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/392969 |
| Acceso en línea: | http://hdl.handle.net/10261/392969 |
| Access Level: | acceso abierto |
| Sumario: | General procedures; establishment and characterization of a simplified in vitro nasal mucosal barrier model using Calu-3 cells; simplified in vitro nasal mucosal barrier model with Calu-3; morphological, histological, and histochemical structure study of the Calu-3 model barrier; cell junction protein immunolabeling; supplementary figures and tables; CS functionalization with sCy5-NHS ester; counterion substitution of DiA and DiD fluorophores; determination of encapsulation efficiency and fluorophore loading; release studies; permeability/transcellular transport studies through the Calu-3 model barrier; preparation of the fluorophore-loaded NCs; excitation and emission spectra of the fluorophores; encapsulation efficiency and fluorophore loading; zeta potential, size, and morphology of the NCs; emission spectra of sCy5@NCs and sCy5@CS-DiA-NCs; FRET signal evaluation by fluorimetry and flow cytometry; NCs stability and NE diffusion through the CS shell in release assays; in vitro cytotoxicity of the NCs; in vitro cellular uptake of the NCs; establishment and characterization of a simplified in vitro nasal mucosal barrier model using Calu-3 cells; nanocapsule transport across the Calu-3 barrier model; and references. |
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