Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador

The thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to stud...

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Autores: Medina Ramírez, Gerardo, Naranjo, Carina, Escobar, Sandra, Araque, Judith, Djabayan, Pablo, Andueza, Félix
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Ecuador
Institución:Universidad Central del Ecuador
Repositorio:Revista FIGEMPA: Investigación y Desarrollo
Idioma:español
OAI Identifier:oai:revistadigital.uce.edu.ec:article/867
Acceso en línea:https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867
Access Level:acceso abierto
Palabra clave:aguas termales
microbiota
resistomas
hot springs
id EC_b5fa91bbfa7f07be434ac533dedaa93a
oai_identifier_str oai:revistadigital.uce.edu.ec:article/867
network_acronym_str EC
network_name_str Ecuador
repository_id_str
dc.title.none.fl_str_mv Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
Microbiota extremofila y resistomas ambientales de la fuente termal “Termas La Merced” Quito-Ecuador
title Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
spellingShingle Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
Medina Ramírez, Gerardo
aguas termales
microbiota
resistomas
hot springs
microbiota
resistomas
title_short Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
title_full Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
title_fullStr Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
title_full_unstemmed Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
title_sort Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-Ecuador
dc.creator.none.fl_str_mv Medina Ramírez, Gerardo
Naranjo, Carina
Escobar, Sandra
Araque, Judith
Djabayan, Pablo
Andueza, Félix
author Medina Ramírez, Gerardo
author_facet Medina Ramírez, Gerardo
Naranjo, Carina
Escobar, Sandra
Araque, Judith
Djabayan, Pablo
Andueza, Félix
author_role author
author2 Naranjo, Carina
Escobar, Sandra
Araque, Judith
Djabayan, Pablo
Andueza, Félix
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv aguas termales
microbiota
resistomas
hot springs
microbiota
resistomas
topic aguas termales
microbiota
resistomas
hot springs
microbiota
resistomas
description The thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to study the water bacterial microbiota of the Termas la Merced Spa, located near the City of Quito, Ecuador, to isolate the bacteria and determine the resistance patterns to antibiotics. Thermal water samples were collected from both the emergency area and the swimming pools used by bathers for a period of 6 months between July and December 2015. The isolate of the mesophilic aerobic bacteria using the Petrifilm ™ plates. Identification of bacterial colonies was performed according to the tests suggested by MacFaddin (2003) and Barrow and Feltham (1993), supplemented with MicrogenTM bacterial identification tests. Antibiotic susceptibility profiles were performed using the Kirby Bauer method. A total of 13 bacterial clones corresponding to Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri and Xenorhabdus beddingii were identified. All isolates showed resistance to at least one antibiotic. The highest number of resistance (4 antibiotics) was exhibited by Brevundimonas diminuta strains. All isolates showed resistance to Cephalothin. It is demonstrated that these waters possess a characteristic microbiota and the presence of environmental resistomas.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867
10.29166/revfig.v1i2.867
url https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867
identifier_str_mv 10.29166/revfig.v1i2.867
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/874
https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5484
https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5483
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/xml
application/zip
dc.publisher.none.fl_str_mv Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador
publisher.none.fl_str_mv Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador
dc.source.none.fl_str_mv FIGEMPA: Investigación y Desarrollo; Vol. 2 No. 2 (2016): WATER; 33 - 37
FIGEMPA: Investigación y Desarrollo; Vol. 2 Núm. 2 (2016): AGUA; 33 - 37
2602-8484
1390-7042
reponame:Revista FIGEMPA: Investigación y Desarrollo
instname:Universidad Central del Ecuador
instacron:UCE
instname_str Universidad Central del Ecuador
instacron_str UCE
institution UCE
reponame_str Revista FIGEMPA: Investigación y Desarrollo
collection Revista FIGEMPA: Investigación y Desarrollo
repository.name.fl_str_mv Revista FIGEMPA: Investigación y Desarrollo - Universidad Central del Ecuador
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spelling Microbiota extremofila and environmental resistomas of the thermal spring “Thermal baths La Merced” Quito-EcuadorMicrobiota extremofila y resistomas ambientales de la fuente termal “Termas La Merced” Quito-EcuadorMedina Ramírez, GerardoNaranjo, CarinaEscobar, SandraAraque, JudithDjabayan, PabloAndueza, Félixaguas termalesmicrobiotaresistomashot springsmicrobiotaresistomasThe thermal waters are not sterile they possess a microbiota reflecting their physicochemical extreme characteristics. There is evidence that this microbiota may be the reservoir of antibiotic resistance genes, termed environmental resistomas. Therefore, the objective of the present work was to study the water bacterial microbiota of the Termas la Merced Spa, located near the City of Quito, Ecuador, to isolate the bacteria and determine the resistance patterns to antibiotics. Thermal water samples were collected from both the emergency area and the swimming pools used by bathers for a period of 6 months between July and December 2015. The isolate of the mesophilic aerobic bacteria using the Petrifilm ™ plates. Identification of bacterial colonies was performed according to the tests suggested by MacFaddin (2003) and Barrow and Feltham (1993), supplemented with MicrogenTM bacterial identification tests. Antibiotic susceptibility profiles were performed using the Kirby Bauer method. A total of 13 bacterial clones corresponding to Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri and Xenorhabdus beddingii were identified. All isolates showed resistance to at least one antibiotic. The highest number of resistance (4 antibiotics) was exhibited by Brevundimonas diminuta strains. All isolates showed resistance to Cephalothin. It is demonstrated that these waters possess a characteristic microbiota and the presence of environmental resistomas.Las aguas termales no son estériles, poseen una microbiota reflejo de sus características fisicoquímicas extremas. Existen evidencias que esta microbiota puede ser el reservorio de genes de resistencia a los antibióticos, denominados resistomas ambientales. Por ello, el objetivo del presente trabajo fue estudiar la microbiota bacteriana del agua del Balneario Termas la Merced, ubicado cerca de la Ciudad de Quito, Ecuador, con el fin de aislar e identificar las bacterias presentes y determinar sus patrones de resistencia a los antibióticos. Se recolectaron muestras de agua termal, tanto de la zona de emergencia, como de las piscinas utilizadas por los bañistas durante los meses de julio a diciembre del 2015. Se realizó el aislamiento bacteriano, utilizando las placas de PetrifilmTM, para bacterias aerobias mesófilas La identificación de las colonias bacterianas se realizó de acuerdo a las pruebas sugeridas por MacFaddin (2003) y Barrow y Feltham (1993), suplementadas con las pruebas de identificación bacteriano MicrogenTM. Los perfiles de susceptibilidad a antibióticos se realizaron utilizando el método de Kirby Bauer. Se identificaron 13 clones bacterianos, correspondientes a las especies Brevundimonas diminuta, Aeromonas schubertii, Budvicia aquatica, Acinetobacter haemolyticus, Bacillus spp, Citrobacter amalonaticus, Pseudomonas stutzeri y Xenorhabdus beddingii. Todos los aislados mostraron resistencia al menos a un antibiótico. El mayor número de resistencias (4 antibióticos) lo exhibieron cepas de la especie Brevundimonas diminuta. Todos los aislados mostraron resistencia a la Cefalotina. Se demuestra que estas aguas poseen una microbiota bacteriana característica y la presencia de resistomas ambientales.Facultad de Ingeniería en Geología, Minas, Petróleos y Ambiental - Universidad Central del Ecuador2016-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/xmlapplication/ziphttps://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/86710.29166/revfig.v1i2.867FIGEMPA: Investigación y Desarrollo; Vol. 2 No. 2 (2016): WATER; 33 - 37FIGEMPA: Investigación y Desarrollo; Vol. 2 Núm. 2 (2016): AGUA; 33 - 372602-84841390-7042reponame:Revista FIGEMPA: Investigación y Desarrolloinstname:Universidad Central del Ecuadorinstacron:UCEspahttps://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/874https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5484https://revistadigital.uce.edu.ec/index.php/RevFIG/article/view/867/5483Derechos de autor 2016 Gerardo Medina Ramírez, Carina Naranjo, Sandra Escobar, Judith Araque, Pablo Djabayan, Félix Anduezahttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess2023-04-10T15:59:32Zoai:revistadigital.uce.edu.ec:article/867Portal de revistashttps://revistadigital.uce.edu.ec/Universidad públicahttps://uce.edu.ec/**Ecuador*602-84841390-7042opendoar:02023-04-10T15:59:32Revista FIGEMPA: Investigación y Desarrollo - Universidad Central del Ecuadorfalse
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