PROGRESS in vitro PROPAGATION Swietenia Macrophylla King (MAHOGANY)
Mahogany (Swietenia macrophylla), is a widely used timber tree species valued for its hardness, strength, beauty and quality. Intensive explotation and inadequate use of this species have resulted in the loss of genetic variability, making impossible the implementation of breeding programs of mahoga...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Ecuador |
| Institución: | Universidad Técnica Estatal de Quevedo |
| Repositorio: | Revista Ciencia y Tecnología |
| Idioma: | inglés |
| OAI Identifier: | oai:revistas.uteq.edu.ec:article/133 |
| Acceso en línea: | https://revistas.uteq.edu.ec/index.php/cyt/article/view/133 |
| Access Level: | acceso abierto |
| Palabra clave: | PROTOCOLO PROPAGACIÓN IN VITRO SWIETENIA MACROPHYLLA SEGMENTOS NODALES. PROTOCOL IN VITRO PROPAGATION NODAL SEGMENTS. |
| Sumario: | Mahogany (Swietenia macrophylla), is a widely used timber tree species valued for its hardness, strength, beauty and quality. Intensive explotation and inadequate use of this species have resulted in the loss of genetic variability, making impossible the implementation of breeding programs of mahogany in Ecuador. In vitro culture is a promising technique that would help reducing this problem by producing plants with high genetic strength. The objective of this research was to establish a method to facilitate in vitro propagation of mahogany from nodal segments. In order to prevent contamination, nodal segments were treated with Ca(ClO)2 solution for 20 min. Healthy explants were transferred to MS/2 culture medium with different concentrations of benzylaminopurine (BAP) and indolebutyric acid (IBA) for further in vitro proliferation, obtaining 70% of shoots on medium supplemented with 2 mg L-1 of BAP and 1 mg L-1 IBA. Bud treatment with different concentrations of ANA for in vitro rooting was not efficient, although the greatest survival rate (65%) was obtained on culture mediumcontaining 2 mg L-1 BAP and 1 mg L-1 NAA. |
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