Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences

A species complex is a group of closely related species, where intraspecific variability overlaps with interspecific variation. Frequently, members of a species complex do not have complete reproductive isolation, therefore, the complex may go through extensive gene flow. In the Caribbean Sea, some...

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Autor: Escobar Ardila, Dairo Alexander
Tipo de recurso: tesis de maestría
Estado:Versión publicada
Fecha de publicación:2010
País:Colombia
Institución:Universidad de los Andes
Repositorio:Séneca: repositorio Uniandes
Idioma:inglés
OAI Identifier:oai:repositorio.uniandes.edu.co:1992/11354
Acceso en línea:http://hdl.handle.net/1992/11354
Access Level:acceso abierto
Palabra clave:Esponjas (Zoología) - Investigaciones
Cliona viridis - Investigaciones
Genética animal - Investigaciones
Filogenia - Investigaciones
Biología
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spelling Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequencesEscobar Ardila, Dairo AlexanderEsponjas (Zoología) - InvestigacionesCliona viridis - InvestigacionesGenética animal - InvestigacionesFilogenia - InvestigacionesBiologíaA species complex is a group of closely related species, where intraspecific variability overlaps with interspecific variation. Frequently, members of a species complex do not have complete reproductive isolation, therefore, the complex may go through extensive gene flow. In the Caribbean Sea, some encrusting and excavating sponges of the genus Cliona (Porifera, Hadromerida, Clionaide) are grouped within the great Cliona viridis complex because of its morphological similarities. This study examined the evolutionary relationships of the Caribbean members of the "Cliona viridis complex" (C. caribbaea, C. tenuis, C. aprica, and C. varians) and related taxa based on nuclear (ITSI and ITS2) and mitochondrial (3' end of ND6) sequences. The intragenomic ITS variation and its secondary structures were evaluated using a mixed approach of Denaturing Gradient Gel Electrophoresis (DGGE), DNA sequencing and secondary structure prediction. Considerable intragenomic variation was found in all the species, with apparently functional ITSI and ITS2 secondary structures. Despite the subtle morphological differentiation in these excavating sponges, the intragenomic copies of C. caribbaea, C. tenuis and C. aprica had a polyphyletic placement In the ITSI and ITS2 genealogies and very Iow divergence Therefore, it is clear that these species constitute a species complex (Ct-complex). "The only exception was C. varians, which presented a monophyletic clade and a group of putative specimens of Pione. These species were the closest Sister groups to the Ct-complex. Genetic distances revealed that part of the interspecific variation overlapped with intraespecific variation, which suggest either incomplete lineage sorting or extensive gene flow within the Ct-complex. Additionally our results support a monophyletic group with C. laticavicola and C. delitrix, but absence of reciprocal monophyly in the species, suggests their recent divergence. Our data shows that the 3' end of ND6, mitochondrial gene was highly conserved and not suitable for phylogenetic analysis at the interspecific level.Magíster en Ciencias BiológicasMaestríaUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Ciencias BiológicasSánchez Muñoz, Juan Armando2010Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_bdcchttp://purl.org/coar/version/c_ab4af688f83e57aaTexthttp://purl.org/redcol/resource_type/TM36 hojasapplication/pdfapplication/pdfhttp://hdl.handle.net/1992/11354u442555.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/reponame:Séneca: repositorio Uniandesinstname:Universidad de los Andesinstacron:Universidad de los AndesengAl consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf22024-11-14T14:22:10Z
dc.title.none.fl_str_mv Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
title Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
spellingShingle Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
Escobar Ardila, Dairo Alexander
Esponjas (Zoología) - Investigaciones
Cliona viridis - Investigaciones
Genética animal - Investigaciones
Filogenia - Investigaciones
Biología
title_short Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
title_full Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
title_fullStr Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
title_full_unstemmed Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
title_sort Phylogenetic relationships among the Caribbean members of the Cliona Viridis complex (Porífera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences
dc.creator.none.fl_str_mv Escobar Ardila, Dairo Alexander
author Escobar Ardila, Dairo Alexander
author_facet Escobar Ardila, Dairo Alexander
author_role author
dc.contributor.none.fl_str_mv Sánchez Muñoz, Juan Armando
dc.subject.none.fl_str_mv Esponjas (Zoología) - Investigaciones
Cliona viridis - Investigaciones
Genética animal - Investigaciones
Filogenia - Investigaciones
Biología
topic Esponjas (Zoología) - Investigaciones
Cliona viridis - Investigaciones
Genética animal - Investigaciones
Filogenia - Investigaciones
Biología
description A species complex is a group of closely related species, where intraspecific variability overlaps with interspecific variation. Frequently, members of a species complex do not have complete reproductive isolation, therefore, the complex may go through extensive gene flow. In the Caribbean Sea, some encrusting and excavating sponges of the genus Cliona (Porifera, Hadromerida, Clionaide) are grouped within the great Cliona viridis complex because of its morphological similarities. This study examined the evolutionary relationships of the Caribbean members of the "Cliona viridis complex" (C. caribbaea, C. tenuis, C. aprica, and C. varians) and related taxa based on nuclear (ITSI and ITS2) and mitochondrial (3' end of ND6) sequences. The intragenomic ITS variation and its secondary structures were evaluated using a mixed approach of Denaturing Gradient Gel Electrophoresis (DGGE), DNA sequencing and secondary structure prediction. Considerable intragenomic variation was found in all the species, with apparently functional ITSI and ITS2 secondary structures. Despite the subtle morphological differentiation in these excavating sponges, the intragenomic copies of C. caribbaea, C. tenuis and C. aprica had a polyphyletic placement In the ITSI and ITS2 genealogies and very Iow divergence Therefore, it is clear that these species constitute a species complex (Ct-complex). "The only exception was C. varians, which presented a monophyletic clade and a group of putative specimens of Pione. These species were the closest Sister groups to the Ct-complex. Genetic distances revealed that part of the interspecific variation overlapped with intraespecific variation, which suggest either incomplete lineage sorting or extensive gene flow within the Ct-complex. Additionally our results support a monophyletic group with C. laticavicola and C. delitrix, but absence of reciprocal monophyly in the species, suggests their recent divergence. Our data shows that the 3' end of ND6, mitochondrial gene was highly conserved and not suitable for phylogenetic analysis at the interspecific level.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv Trabajo de grado - Maestría
info:eu-repo/semantics/masterThesis
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dc.identifier.none.fl_str_mv http://hdl.handle.net/1992/11354
u442555.pdf
instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
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url http://hdl.handle.net/1992/11354
identifier_str_mv u442555.pdf
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dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/openAccess
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application/pdf
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dc.publisher.none.fl_str_mv Uniandes
Maestría en Ciencias Biológicas
Facultad de Ciencias
Departamento de Ciencias Biológicas
publisher.none.fl_str_mv Uniandes
Maestría en Ciencias Biológicas
Facultad de Ciencias
Departamento de Ciencias Biológicas
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