Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture

ABSTRACT: The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteiniza...

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Detalles Bibliográficos
Autores: Ramírez Cadavid, David Alexander, Escobar Sierra, Diana Marcela, Ossa Orozco, Claudia Patricia, Rojas Vahos, Diego Fernando, Zapata Ocampo, Paola Andrea, Atehortúa Garcés, Lucía
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Colombia
Institución:Universidad de Antioquia
Repositorio:Repositorio UdeA
Idioma:inglés
OAI Identifier:oai:bibliotecadigital.udea.edu.co:10495/7717
Acceso en línea:http://hdl.handle.net/10495/7717
Access Level:acceso abierto
Palabra clave:Ganoderma lucidum
Métodos de extracción
Quitina
id CO_95df994c677603e4e404693d1e38bdf9
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network_acronym_str CO
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repository_id_str
spelling Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged cultureRamírez Cadavid, David AlexanderEscobar Sierra, Diana MarcelaOssa Orozco, Claudia PatriciaRojas Vahos, Diego FernandoZapata Ocampo, Paola AndreaAtehortúa Garcés, LucíaGanoderma lucidumMétodos de extracciónQuitinaABSTRACT: The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g−1 (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.Hindawi Publishing CorporationGrupo de Investigación en BiomaterialesEgipto2017-07-18T19:13:18Z2017-07-18T19:13:18Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigación5application/pdfapplication/pdfOspina, S. P., Ramírez, D. A., Escobar, D. M., Ossa, C. P., Rojas, D. F., Zapata, P. & Atehortúa, L. (2014).Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture. BioMed Research International, 2014, 1-6. DOI: 10.1155/2014/1690712314-6133http://hdl.handle.net/10495/771710.1155/2014/1690712314-6141engAtribución 2.5 Colombia (CC BY 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/reponame:Repositorio UdeAinstname:Universidad de Antioquiainstacron:Universidad de Antioquia2021-05-29T20:30:25Z
dc.title.none.fl_str_mv Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
title Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
spellingShingle Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
Ramírez Cadavid, David Alexander
Ganoderma lucidum
Métodos de extracción
Quitina
title_short Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
title_full Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
title_fullStr Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
title_full_unstemmed Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
title_sort Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
dc.creator.none.fl_str_mv Ramírez Cadavid, David Alexander
Escobar Sierra, Diana Marcela
Ossa Orozco, Claudia Patricia
Rojas Vahos, Diego Fernando
Zapata Ocampo, Paola Andrea
Atehortúa Garcés, Lucía
author Ramírez Cadavid, David Alexander
author_facet Ramírez Cadavid, David Alexander
Escobar Sierra, Diana Marcela
Ossa Orozco, Claudia Patricia
Rojas Vahos, Diego Fernando
Zapata Ocampo, Paola Andrea
Atehortúa Garcés, Lucía
author_role author
author2 Escobar Sierra, Diana Marcela
Ossa Orozco, Claudia Patricia
Rojas Vahos, Diego Fernando
Zapata Ocampo, Paola Andrea
Atehortúa Garcés, Lucía
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ganoderma lucidum
Métodos de extracción
Quitina
topic Ganoderma lucidum
Métodos de extracción
Quitina
description ABSTRACT: The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g−1 (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.
publishDate 2014
dc.date.none.fl_str_mv 2014
2017-07-18T19:13:18Z
2017-07-18T19:13:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_2df8fbb1
https://purl.org/redcol/resource_type/ART
Artículo de investigación
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Ospina, S. P., Ramírez, D. A., Escobar, D. M., Ossa, C. P., Rojas, D. F., Zapata, P. & Atehortúa, L. (2014).Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture. BioMed Research International, 2014, 1-6. DOI: 10.1155/2014/169071
2314-6133
http://hdl.handle.net/10495/7717
10.1155/2014/169071
2314-6141
identifier_str_mv Ospina, S. P., Ramírez, D. A., Escobar, D. M., Ossa, C. P., Rojas, D. F., Zapata, P. & Atehortúa, L. (2014).Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture. BioMed Research International, 2014, 1-6. DOI: 10.1155/2014/169071
2314-6133
10.1155/2014/169071
2314-6141
url http://hdl.handle.net/10495/7717
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv Atribución 2.5 Colombia (CC BY 2.5 CO)
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Atribución 2.5 Colombia (CC BY 2.5 CO)
https://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 5
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
Grupo de Investigación en Biomateriales
Egipto
publisher.none.fl_str_mv Hindawi Publishing Corporation
Grupo de Investigación en Biomateriales
Egipto
dc.source.none.fl_str_mv reponame:Repositorio UdeA
instname:Universidad de Antioquia
instacron:Universidad de Antioquia
instname_str Universidad de Antioquia
instacron_str Universidad de Antioquia
institution Universidad de Antioquia
reponame_str Repositorio UdeA
collection Repositorio UdeA
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score 15,812455

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