HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells
Even with sustained antiretroviral therapy, resting CD4 + T cells remain a persistent reservoir of HIV infection, representing a critical barrier to curing HIV. Here, we demonstrate that CD8 + T cells recognize infected, non-activated CD4 + T cells in the absence of de novo protein production, as me...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Colombia |
| Institución: | Universidad del Rosario |
| Repositorio: | Repositorio EdocUR - U. Rosario |
| Idioma: | inglés |
| OAI Identifier: | oai:repository.urosario.edu.co:10336/23642 |
| Acceso en línea: | https://doi.org/10.1016/j.celrep.2019.03.016 https://repository.urosario.edu.co/handle/10336/23642 |
| Access Level: | acceso abierto |
| Palabra clave: | T lymphocyte receptor Antigen presentation Article CD4+ T lymphocyte CD8+ T lymphocyte Colorimetry Controlled study Cytokine production Degranulation Flow cytometry Fluorescence microscopy Fluorescence resonance energy transfer Human Human cell Human immunodeficiency virus 1 infection Immune response Immunological synapse Long terminal repeat Molecular recognition Priority journal Protein cleavage Protein protein interaction Synapse Virus entry Virus genome Virus particle Cytotoxic T lymphocytes Elite controllers Granzyme HIV HIV cure HLA Immunologic synapse Perforin |
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HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T CellsMonel, BlandineMcKeon, AnnmarieLamothe-Molina, PedroJani, PriyaBoucau, JulieJones, R. BradLe Gall, SylvieWalker, Bruce D.Pacheco Nieva, YovanaT lymphocyte receptorAntigen presentationArticleCD4+ T lymphocyteCD8+ T lymphocyteColorimetryControlled studyCytokine productionDegranulationFlow cytometryFluorescence microscopyFluorescence resonance energy transferHumanHuman cellHuman immunodeficiency virus 1 infectionImmune responseImmunological synapseLong terminal repeatMolecular recognitionPriority journalProtein cleavageProtein protein interactionSynapseVirus entryVirus genomeVirus particleCytotoxic T lymphocytesElite controllersGranzymeHIVHIV cureHLAImmunologic synapsePerforinEven with sustained antiretroviral therapy, resting CD4 + T cells remain a persistent reservoir of HIV infection, representing a critical barrier to curing HIV. Here, we demonstrate that CD8 + T cells recognize infected, non-activated CD4 + T cells in the absence of de novo protein production, as measured by immune synapse formation, degranulation, cytokine production, and killing of infected cells. Immune recognition is induced by HLA-I presentation of peptides derived from incoming viral particles, and recognition occurred either following cell-free virus infection or following cell-to-cell spread. CD8 + T cells from HIV controllers mediate more effective immune recognition than CD8 + T cells from progressors. These results indicate that non-activated HIV-infected CD4 + T cells can be targeted by CD8 + T cells directly after HIV entry, before reverse transcription, and thus before the establishment of latency, and suggest a mechanism whereby the immune response may reduce the size of the HIV reservoir. The cure for HIV is not achievable due to HIV reservoirs, mostly in resting CD4 + T cells. Monel et al. show that CD8 + T cells from HIV controllers are able to establish immunological synapses with HIV + resting CD4 + T cells, leading to IFN-?, MIP1-? production, degranulation, and the elimination of the target cells. © 2019 The AuthorsElsevier B.V.20192020-05-26T00:03:57Zinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.celrep.2019.03.01622111247https://repository.urosario.edu.co/handle/10336/23642reponame:Repositorio EdocUR - U. Rosarioinstname:Universidad del Rosarioinstacron:Universidad del Rosarioenghttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85063383455&doi=10.1016%2fj.celrep.2019.03.016&partnerID=40&md5=332d50ba72513d6eed03a3e1bef75e1cinfo:eu-repo/semantics/openAccess2022-05-02T07:37:21Z |
| dc.title.none.fl_str_mv |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| title |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| spellingShingle |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells Monel, Blandine T lymphocyte receptor Antigen presentation Article CD4+ T lymphocyte CD8+ T lymphocyte Colorimetry Controlled study Cytokine production Degranulation Flow cytometry Fluorescence microscopy Fluorescence resonance energy transfer Human Human cell Human immunodeficiency virus 1 infection Immune response Immunological synapse Long terminal repeat Molecular recognition Priority journal Protein cleavage Protein protein interaction Synapse Virus entry Virus genome Virus particle Cytotoxic T lymphocytes Elite controllers Granzyme HIV HIV cure HLA Immunologic synapse Perforin |
| title_short |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| title_full |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| title_fullStr |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| title_full_unstemmed |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| title_sort |
HIV Controllers Exhibit Effective CD8 + T Cell Recognition of HIV-1-Infected Non-activated CD4 + T Cells |
| dc.creator.none.fl_str_mv |
Monel, Blandine McKeon, Annmarie Lamothe-Molina, Pedro Jani, Priya Boucau, Julie Jones, R. Brad Le Gall, Sylvie Walker, Bruce D. Pacheco Nieva, Yovana |
| author |
Monel, Blandine |
| author_facet |
Monel, Blandine McKeon, Annmarie Lamothe-Molina, Pedro Jani, Priya Boucau, Julie Jones, R. Brad Le Gall, Sylvie Walker, Bruce D. Pacheco Nieva, Yovana |
| author_role |
author |
| author2 |
McKeon, Annmarie Lamothe-Molina, Pedro Jani, Priya Boucau, Julie Jones, R. Brad Le Gall, Sylvie Walker, Bruce D. Pacheco Nieva, Yovana |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
T lymphocyte receptor Antigen presentation Article CD4+ T lymphocyte CD8+ T lymphocyte Colorimetry Controlled study Cytokine production Degranulation Flow cytometry Fluorescence microscopy Fluorescence resonance energy transfer Human Human cell Human immunodeficiency virus 1 infection Immune response Immunological synapse Long terminal repeat Molecular recognition Priority journal Protein cleavage Protein protein interaction Synapse Virus entry Virus genome Virus particle Cytotoxic T lymphocytes Elite controllers Granzyme HIV HIV cure HLA Immunologic synapse Perforin |
| topic |
T lymphocyte receptor Antigen presentation Article CD4+ T lymphocyte CD8+ T lymphocyte Colorimetry Controlled study Cytokine production Degranulation Flow cytometry Fluorescence microscopy Fluorescence resonance energy transfer Human Human cell Human immunodeficiency virus 1 infection Immune response Immunological synapse Long terminal repeat Molecular recognition Priority journal Protein cleavage Protein protein interaction Synapse Virus entry Virus genome Virus particle Cytotoxic T lymphocytes Elite controllers Granzyme HIV HIV cure HLA Immunologic synapse Perforin |
| description |
Even with sustained antiretroviral therapy, resting CD4 + T cells remain a persistent reservoir of HIV infection, representing a critical barrier to curing HIV. Here, we demonstrate that CD8 + T cells recognize infected, non-activated CD4 + T cells in the absence of de novo protein production, as measured by immune synapse formation, degranulation, cytokine production, and killing of infected cells. Immune recognition is induced by HLA-I presentation of peptides derived from incoming viral particles, and recognition occurred either following cell-free virus infection or following cell-to-cell spread. CD8 + T cells from HIV controllers mediate more effective immune recognition than CD8 + T cells from progressors. These results indicate that non-activated HIV-infected CD4 + T cells can be targeted by CD8 + T cells directly after HIV entry, before reverse transcription, and thus before the establishment of latency, and suggest a mechanism whereby the immune response may reduce the size of the HIV reservoir. The cure for HIV is not achievable due to HIV reservoirs, mostly in resting CD4 + T cells. Monel et al. show that CD8 + T cells from HIV controllers are able to establish immunological synapses with HIV + resting CD4 + T cells, leading to IFN-?, MIP1-? production, degranulation, and the elimination of the target cells. © 2019 The Authors |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2020-05-26T00:03:57Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://doi.org/10.1016/j.celrep.2019.03.016 22111247 https://repository.urosario.edu.co/handle/10336/23642 |
| url |
https://doi.org/10.1016/j.celrep.2019.03.016 https://repository.urosario.edu.co/handle/10336/23642 |
| identifier_str_mv |
22111247 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063383455&doi=10.1016%2fj.celrep.2019.03.016&partnerID=40&md5=332d50ba72513d6eed03a3e1bef75e1c |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
reponame:Repositorio EdocUR - U. Rosario instname:Universidad del Rosario instacron:Universidad del Rosario |
| instname_str |
Universidad del Rosario |
| instacron_str |
Universidad del Rosario |
| institution |
Universidad del Rosario |
| reponame_str |
Repositorio EdocUR - U. Rosario |
| collection |
Repositorio EdocUR - U. Rosario |
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1825051768237588480 |
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15,81155 |