Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab

The identification of exclusive markers to target HIV-reservoir cells will represent a significant advance in the search for therapies to cure HIV. Here, we identify the B lymphocyte antigen CD20 as a marker for HIV-infected cells in vitro and in vivo. The CD20 molecule is dimly expressed in a subpo...

Descripción completa

Detalles Bibliográficos
Autores: Serra-Peinado, Carla|||0000-0003-1413-3990, Grau Expósito, Judith|||0000-0001-9350-2429, Luque-Ballesteros, Laura|||0000-0001-9515-5889, Astorga-Gamaza, Antonio|||0000-0002-1276-5276, Navarro, Jordi|||0000-0002-7187-0367, Gallego-Rodriguez, Jenny, Martín Castillo, Mario|||0000-0001-6435-0374, Curran, Adrian|||0000-0002-1263-0814, Burgos, Joaquín|||0000-0001-8445-3047, Ribera, Esteban|||0000-0002-3752-8808, Raventós, Berta|||0000-0002-4668-2970, Willekens, Rein|||0000-0001-7791-3682, Torrella Domingo, Adriana|||0000-0003-1848-5096, Planas, Bibiana|||0000-0002-1490-7127, Badía, Rosa|||0000-0002-8222-5697, García, Felipe|||0000-0001-7658-5832, Castellvi, Josep|||0000-0001-5745-9996, Genescà Ferrer, Meritxell|||0000-0001-6413-3812, Falcó, Vicenç|||0000-0001-9626-0023, Buzón, Maria José|||0000-0003-4427-9413
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:223671
Acceso en línea:https://ddd.uab.cat/record/223671
https://dx.doi.org/urn:doi:10.1038/s41467-019-11556-4
Access Level:acceso abierto
Palabra clave:Anti-HIV Agents
Antigens, CD20
CD4-Positive T-Lymphocytes
Flow Cytometry
HIV Infections
HIV-1
Humans
Immunologic Factors
Immunologic Memory
Leukocytes, Mononuclear
Lymph Nodes
Lymphocyte Activation
Rituximab
RNA, Viral
Virus Activation
Virus Latency
Descripción
Sumario:The identification of exclusive markers to target HIV-reservoir cells will represent a significant advance in the search for therapies to cure HIV. Here, we identify the B lymphocyte antigen CD20 as a marker for HIV-infected cells in vitro and in vivo. The CD20 molecule is dimly expressed in a subpopulation of CD4-positive (CD4) T lymphocytes from blood, with high levels of cell activation and heterogeneous memory phenotypes. In lymph node samples from infected patients, CD20 is present in productively HIV-infected cells, and ex vivo viral infection selectively upregulates the expression of CD20 during early infection. In samples from patients on antiretroviral therapy (ART) this subpopulation is significantly enriched in HIV transcripts, and the anti-CD20 monoclonal antibody Rituximab induces cell killing, which reduces the pool of HIV-expressing cells when combined with latency reversal agents. We provide a tool for targeting this active HIV-reservoir after viral reactivation in patients while on ART.

Similares en LA Referencia