Rol de la proteína Mitofusina 2 en la fagocitosis de células de Sertoli de la línea celular de ratón 42GPA9
Sertoli cells have multiple roles in germ cell development, ranging from physical support to supply of nutrients. The mechanisms that regulates Sertoli cell metabolism are central to the maintenance of spermatogenesis and male fertility. One of the functions of Sertoli cells corresponds to phagocyto...
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| Tipo de documento: | tese |
| Estado: | Versão publicada |
| Data de publicação: | 2018 |
| País: | Chile |
| OAI Identifier: | oai:repositorio.anid.cl:10533/246471 |
| Acesso em linha: | https://hdl.handle.net/10533/246471 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Ciencias Naturales Otras Ciencias Naturales |
| Resumo: | Sertoli cells have multiple roles in germ cell development, ranging from physical support to supply of nutrients. The mechanisms that regulates Sertoli cell metabolism are central to the maintenance of spermatogenesis and male fertility. One of the functions of Sertoli cells corresponds to phagocytosis of residual bodies and apoptotic spermatogenic cells. It has been shown in macrophages that there is a positive correlation between mitochondrial membrane potential and the engulfment capacity, indicating that mitochondria have a role in phagocytosis preservation. Mitochondria are dynamic organelles that continuously undergo fission and fusion, which are necessary for the maintenance of mitochondrial homeostasis. Mitofusin 2 (Mfn2) is a mitochondrial outer membrane protein involved in the rearrangement of these organelles through the regulation of the fusion process, besides playing an impoprtant role in mitochondrial metabolism, quality control and in the modulation of ER-mitochondria contacts, among others. In this work we evaluated the role of Mfn2 in the maintenance of mitochondrial and phagocytic function in Sertoli cells. We generated a Sertoli 42GPA9 Mitofusin 2 knockdown (KD) cell line and evaluated different parameters of mitochondrial function and observed an increase in ROS production, a decrease in mitochondrial membrane potential, higher calcium levels and a fragmented mitochondrial morphology, demonstrating an altered mitochondrial function. Phagocytosis was determined by incubating the cells with fluorescent latex beads and evaluating its internalization by microscopy and flow cytometry, which was reduced in a 30%. We also observed that control cells (scramble) are more sensitive to Dibutyryl-cAMP showing a higher phagocytosis inhibition compared to Mfn 2 knockdown Sertoli cells. These results suggest that Mfn2 is involved in Sertoli cell phagocytosis by a yet unknown mechanism. |
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