STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL

It was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H2O2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delign...

Descripción completa

Detalles Bibliográficos
Autores: Lucarini, Adriana Célia, Delquiaro, Ana Carolina Tezotto, Vidoca, Ludmila Carolina Prado Tavares, Braz, Rafaela, Martins, Roberta Morelli, Alves, Taís Pereira
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:The Journal of Engineering and Exact Sciences
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.ufv.br:article/2317
Acceso en línea:https://periodicos.ufv.br/jcec/article/view/2446941603022017242
Access Level:acceso abierto
Palabra clave:Ethanol
Biomass
Lignocellulosics
Enzymatic Hydrolisis
Cellulase
Etanol
Biomassa
Lignocelulósica
Hidrólise enzimática
Celulase
Descripción
Sumario:It was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H2O2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delignified it was observed the removal of 50% of the lignin content. The enzymatic activity of T. reesei cellulase and commercial Cellic® CTec2 was quantified. The reaction pH, enzyme concentration and reaction time were studied, obtaining optimum pH 4.0 and 5.5 for T. reesei and Cellic® CTec2, respectively. The highest yield in terms of enzyme concentration was 10 FPU/g of straw for both enzymes and for the time, both enzymes reduced conversion after 24 hours of hydrolysis, possibly due to microbial contamination. Finally, it has been carried out a material balance for the commercial cellulase, resulting in a yield of the enzymatic hydrolysis of the cellulose content of 23.8%.