A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions

Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured...

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Detalles Bibliográficos
Autores: Almodin, Carlos Gilberto [UNIFESP], Moron, Antonio Fernandes [UNIFESP], Kulay Junior, Luiz [UNIFESP], Minguetti-Camara, Vânia Cibele, Moraes, Andréa Cristina [UNIFESP], Torloni, Maria Regina [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/28474
Acceso en línea:http://dx.doi.org/10.1016/j.fertnstert.2005.02.051
http://repositorio.unifesp.br/handle/11600/28474
Access Level:acceso abierto
Palabra clave:PGD
training
bovine
embryos
model
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oai_identifier_str oai:repositorio.unifesp.br:11600/28474
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactionsPGDtrainingbovineembryosmodelObjective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.Materbaby Reprod Humana & Genet, Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilWeb of ScienceElsevier B.V.Materbaby Reprod Humana & GenetUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:38:04Z2016-01-24T12:38:04Z2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion895-899http://dx.doi.org/10.1016/j.fertnstert.2005.02.051Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005.10.1016/j.fertnstert.2005.02.0510015-0282http://repositorio.unifesp.br/handle/11600/28474WOS:000232542000013ark:/48912/001300001w72nengFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPAlmodin, Carlos Gilberto [UNIFESP]Moron, Antonio Fernandes [UNIFESP]Kulay Junior, Luiz [UNIFESP]Minguetti-Camara, Vânia CibeleMoraes, Andréa Cristina [UNIFESP]Torloni, Maria Regina [UNIFESP]2023-02-15T10:56:37Zoai:repositorio.unifesp.br:11600/28474Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652023-02-15T10:56:37Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
title A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
spellingShingle A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
Almodin, Carlos Gilberto [UNIFESP]
PGD
training
bovine
embryos
model
title_short A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
title_full A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
title_fullStr A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
title_full_unstemmed A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
title_sort A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
dc.creator.none.fl_str_mv Almodin, Carlos Gilberto [UNIFESP]
Moron, Antonio Fernandes [UNIFESP]
Kulay Junior, Luiz [UNIFESP]
Minguetti-Camara, Vânia Cibele
Moraes, Andréa Cristina [UNIFESP]
Torloni, Maria Regina [UNIFESP]
author Almodin, Carlos Gilberto [UNIFESP]
author_facet Almodin, Carlos Gilberto [UNIFESP]
Moron, Antonio Fernandes [UNIFESP]
Kulay Junior, Luiz [UNIFESP]
Minguetti-Camara, Vânia Cibele
Moraes, Andréa Cristina [UNIFESP]
Torloni, Maria Regina [UNIFESP]
author_role author
author2 Moron, Antonio Fernandes [UNIFESP]
Kulay Junior, Luiz [UNIFESP]
Minguetti-Camara, Vânia Cibele
Moraes, Andréa Cristina [UNIFESP]
Torloni, Maria Regina [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Materbaby Reprod Humana & Genet
Universidade Federal de São Paulo (UNIFESP)
dc.subject.por.fl_str_mv PGD
training
bovine
embryos
model
topic PGD
training
bovine
embryos
model
description Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
2016-01-24T12:38:04Z
2016-01-24T12:38:04Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fertnstert.2005.02.051
Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005.
10.1016/j.fertnstert.2005.02.051
0015-0282
http://repositorio.unifesp.br/handle/11600/28474
WOS:000232542000013
dc.identifier.dark.fl_str_mv ark:/48912/001300001w72n
url http://dx.doi.org/10.1016/j.fertnstert.2005.02.051
http://repositorio.unifesp.br/handle/11600/28474
identifier_str_mv Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005.
10.1016/j.fertnstert.2005.02.051
0015-0282
WOS:000232542000013
ark:/48912/001300001w72n
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility and Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 895-899
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15.198674

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