A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/28474 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.fertnstert.2005.02.051 http://repositorio.unifesp.br/handle/11600/28474 |
| Access Level: | acceso abierto |
| Palabra clave: | PGD training bovine embryos model |
| Sumario: | Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis. |
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