Comparison of analytical methods in the determination of lipids and polyunsaturated fatty acids by gas chromatography in infant formula
Long chain polyunsaturated fatty acids are involved in several physiological and metabolic processes of human organism. Considering the fatty acids as crucial compound in the infantile nutrition, they have been supplemented in infant formula to substitute maternal milk. For quantifying the long chai...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Brasil |
| Institución: | Instituto Adolfo Lutz |
| Repositorio: | Revista do Instituto Adolfo Lutz (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/32737 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/RIAL/article/view/32737 |
| Access Level: | acceso abierto |
| Palabra clave: | polyunsaturated fatty acid infantile formula analytical methodology gas chromatography lipids ácidos graxos polinsaturados fórmula infantil método analítico cromatografia gasosa lipídeos |
| Sumario: | Long chain polyunsaturated fatty acids are involved in several physiological and metabolic processes of human organism. Considering the fatty acids as crucial compound in the infantile nutrition, they have been supplemented in infant formula to substitute maternal milk. For quantifying the long chain polyunsaturated fatty acids requires a fat extraction process in mild conditions, due to the occurrence of reactive sites in their molecules. In the present work the analytical methods for determining the polyunsaturated fatty acid and lipids in infantile formula were compared. For these purposes, a sample of infantile formula from the National Institute of Standards and Technology was analyzed, following methodologies described in the “Normas Analíticas do Instituto Adolfo Lutz” and the Association of Official Analytical Chemistry (AOAC).The polyunsaturated fatty acid contents were assessed by using distinct internal standards and correction factors for flame ionization detector. Significant differences (p<0.05) were found among the total lipids and polyunsaturated fatty acids results, in infantile formula analyzed on different lipid extraction methods. The lowest dispersions results (rsd %), of polyunsaturated fatty acid were observed by the AOAC methodology. According to these data, the use of fatty acid methyl ester 23:0 internal standard and of theoretical correction factors of flame ionization detector in relation to the internal standard itself were the most suitable for determining the polyunsaturated fatty acid. |
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