Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model1

Purpose: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. Methods: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adapti...

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Detalles Bibliográficos
Autores: Meirelles, Rogério José de Azevedo, Neto, Fermino Sanches Lizarte, Cirino, Mucio Luiz de Assis, Novais, Paulo Cezar, Gula, Isabella Stracieri, Silva, Jairo Pinheiro da, Tazzima, Maria de Fátima Galli Sorita, Fazan, Valéria Paula Sassoli, Durand, Marina Toledo, Tirapelli, Daniela Pretti da Cunha [UNESP], de Carvalho, Camila Albuquerque Melo, Schimming, Bruno César [UNESP], Molina, Carlos Augusto Fernandes, Junior, Silvio Tucci, Tirapelli, Luis Fernando
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/201862
Acceso en línea:http://dx.doi.org/10.1590/s0102-865020200030000005
http://hdl.handle.net/11449/201862
Access Level:acceso abierto
Palabra clave:Apoptosis
Erectile Dysfunction
Ethanol
MicroRNAs
Rats
Descripción
Sumario:Purpose: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. Methods: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. Results: Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. Conclusion: Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.

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