Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model

Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The object...

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Detalles Bibliográficos
Autores: Sarraipo, Vagner Schiavoni, Lizarte Neto, Fermino Sanches, Mello de Carvalho, Camila Albuquerque, Silva, Jairo Pinheiro da, Durand, Marina Toledo, Schimming, Bruno Cezar [UNESP], Martinez, Marcelo, Tazima Sorita Galli, Maria de Fatima, Buono, Fabianna de Oliveira, Barbirato da Mata Tiezzi, Maria Fernanda, Novais, Paulo Cezar, Gula, Isabella Stracieri, Thomazini, Jose Antonio, Sassoli Fazan, Valeria Paula, Rodrigues, Andressa Romualdo, Cunha Tirapelli, Daniela Pretti da, Assis Cirino, Mucio Luiz de, Fernandes Molina, Carlos Augusto, Tucci Junior, Silvio, Tirapelli, Luis Fernando
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/209617
Acceso en línea:http://hdl.handle.net/11449/209617
Access Level:acceso abierto
Palabra clave:Alcoholism
Apoptosis
Erectile Dysfunction
MicroRNAs
Descripción
Sumario:Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavemosum of rats submitted to a semivoltmtary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression ofAIF and PARP and their regulatory inicroRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoltmtary alcoholism.

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