Decrease from main root canal and intratubular Fusobacterium nucleatum and its endotoxin after ultrasonic activation of conventional and alternative irrigation solutions

This study evaluated conventional and alternative irrigation solutions combined with ultrasonic irrigant activation (UIA) against Fusobacterium nucleatum (F. nucleatum) and its endotoxin (LPS) in main root canal and dentinal tubules, using a new intratubular infection model. Seventy dentin cylinders...

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Detalhes bibliográficos
Autores: Barros, Mirela Cesar, Feliz Pedrinha, Victor, Eduardo Oliveira, Felipe [UNESP], Marcucci, Maria Cristina [UNESP], Gomes, Brenda Paula F. A., Oliveira, Luciane Dias de [UNESP], Andrade, Flaviana Bombarda de
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/308088
Acesso em linha:http://dx.doi.org/10.1080/08927014.2024.2426765
https://hdl.handle.net/11449/308088
Access Level:acceso abierto
Palavra-chave:Endodontics
Fusobacterium nucleatum
microbiology
root canal irrigants
ultrasound
Descrição
Resumo:This study evaluated conventional and alternative irrigation solutions combined with ultrasonic irrigant activation (UIA) against Fusobacterium nucleatum (F. nucleatum) and its endotoxin (LPS) in main root canal and dentinal tubules, using a new intratubular infection model. Seventy dentin cylinders were infected with F. nucleatum for seven days under anaerobic conditions and treated with 2.5% sodium hypochlorite (NaOCl), limewater + 2.5% NaOCl, and 10% ethanolic propolis extract (EEP), administered by syringe irrigation (SI) or UIA. Microbiological samples were collected before and after irrigation to determine CFU ml–1 and LPS levels. Confocal microscopy assessed bacterial membrane damage with Live/Dead staining. Irrigation solutions effectively reduced CFU ml–1. UIA caused greater damage to the bacterial membranes and reduced LPS levels. The ultrasonic activation of 10% EEP and limewater + 2.5% NaOCl were comparable to 2.5% NaOCl (p > 0.05). UIA improved the effectiveness of solutions, suggesting potential for alternative substances. Randomized clinical trials using these protocols are recommended.

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