Designing silver nanoparticles for detecting levodopa (3,4-dihydroxyphenylalanine, l-dopa) using surface-enhanced raman scattering (SERS)

Detection of the drug Levodopa (3,4-dihydroxyphenylalanine, L-Dopa) is essential for the medical treatment of several neural disorders, including Parkinson’s disease. In this paper, we employed surface-enhanced Raman scattering (SERS) with three shapes of silver nanoparticles (nanostars, AgNS; nanos...

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Detalles Bibliográficos
Autores: Rubira, Rafael Jesus Gonçalves [UNESP], Camacho, Sabrina Alessio [UNESP], Martin, Cibely Silva [UNESP], Mejía-Salazar, Jorge Ricardo, Gómez, Faustino Reyes, da Silva, Robson Rosa, de Oliveira Junior, Osvaldo Novais, Alessio, Priscila [UNESP], Constantino, Carlos José Leopoldo [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/201422
Acceso en línea:http://dx.doi.org/10.3390/s20010015
http://hdl.handle.net/11449/201422
Access Level:acceso abierto
Palabra clave:Ag nanoparticles
L-Dopa
Multidimensional projections
SERS
Descripción
Sumario:Detection of the drug Levodopa (3,4-dihydroxyphenylalanine, L-Dopa) is essential for the medical treatment of several neural disorders, including Parkinson’s disease. In this paper, we employed surface-enhanced Raman scattering (SERS) with three shapes of silver nanoparticles (nanostars, AgNS; nanospheres, AgNP; and nanoplates, AgNPL) to detect L-Dopa in the nanoparticle dispersions. The sensitivity of the L-Dopa SERS signal depended on both nanoparticle shape and L-Dopa concentration. The adsorption mechanisms of L-Dopa on the nanoparticles inferred from a detailed analysis of the Raman spectra allowed us to determine the chemical groups involved. For instance, at concentrations below/equivalent to the limit found in human plasma (between 10−7–10−8 mol/L), L-Dopa adsorbs on AgNP through its ring, while at 10−5–10−6 mol/L adsorption is driven by the amino group. At even higher concentrations, above 10−4 mol/L, L-Dopa polymerization predominates. Therefore, our results show that adsorption depends on both the type of Ag nanoparticles (shape and chemical groups surrounding the Ag surface) and the L-Dopa concentration. The overall strategy based on SERS is a step forward to the design of nanostructures to detect analytes of clinical interest with high specificity and at varied concentration ranges.

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