Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes

Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor for...

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Autores: Gonçalves, Fabiany da Costa, Luk, Franka, Korevaar, Sander S., Bouzid, Rachid, Paz, Ana Helena da Rosa, Iglesias, Carmen López, Baan, Carla C., Merino, Ana, Hoogduijn, Martin J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/175085
Acceso en línea:http://hdl.handle.net/10183/175085
Access Level:acceso abierto
Palabra clave:Células mesenquimais estromais
Imunidade
Tecido adiposo
Immunosuppression
Mesenchymal stem cells
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spelling 2018-04-26T02:33:33Z20172045-2322http://hdl.handle.net/10183/175085001065696Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape. MP exhibited ATPase, nucleotidase and esterase activity, indicating they are enzymatically active. MP and MPγ did not physically interact with T cells and had no effect on CD4+ and CD8+ T cells proliferation. However, MP and MPγ selectively bound to monocytes and decreased the frequency of pro-inflammatory CD14+CD16+ monocytes by induction of selective apoptosis. MP and MPγ increased the percentage of CD90 positive monocytes, and MPγ but not MP increased the percentage of antiinflammatory PD-L1 monocytes. MPγ increased mRNA expression of PD-L1 in monocytes. These data demonstrate that MP have immunomodulatory properties and have potential as a novel cell-free therapy for treatment of immunological disorders.application/pdfengScientific reports. London. Vol. 7 (Sep. 2017), 12100, 13 f.Células mesenquimais estromaisImunidadeTecido adiposoImmunosuppressionMesenchymal stem cellsMembrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSGonçalves, Fabiany da CostaLuk, FrankaKorevaar, Sander S.Bouzid, RachidPaz, Ana Helena da RosaIglesias, Carmen LópezBaan, Carla C.Merino, AnaHoogduijn, Martin J.ORIGINAL001065696.pdf001065696.pdfTexto completo (inglês)application/pdf2263134http://www.lume.ufrgs.br/bitstream/10183/175085/1/001065696.pdf75cdac8cf95ec551ff06e9a3efa7e2cbMD51TEXT001065696.pdf.txt001065696.pdf.txtExtracted Texttext/plain52654http://www.lume.ufrgs.br/bitstream/10183/175085/2/001065696.pdf.txt65ea495ef0838d9165221ef0d930b599MD52THUMBNAIL001065696.pdf.jpg001065696.pdf.jpgGenerated Thumbnailimage/jpeg2011http://www.lume.ufrgs.br/bitstream/10183/175085/3/001065696.pdf.jpgbaf8588aae9a26e3d8533f45a2ea4779MD5310183/1750852024-05-01 06:51:55.985434oai:www.lume.ufrgs.br:10183/175085Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2024-05-01T09:51:55Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
title Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
spellingShingle Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
Gonçalves, Fabiany da Costa
Células mesenquimais estromais
Imunidade
Tecido adiposo
Immunosuppression
Mesenchymal stem cells
title_short Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
title_full Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
title_fullStr Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
title_full_unstemmed Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
title_sort Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
dc.creator.none.fl_str_mv Gonçalves, Fabiany da Costa
Luk, Franka
Korevaar, Sander S.
Bouzid, Rachid
Paz, Ana Helena da Rosa
Iglesias, Carmen López
Baan, Carla C.
Merino, Ana
Hoogduijn, Martin J.
author Gonçalves, Fabiany da Costa
author_facet Gonçalves, Fabiany da Costa
Luk, Franka
Korevaar, Sander S.
Bouzid, Rachid
Paz, Ana Helena da Rosa
Iglesias, Carmen López
Baan, Carla C.
Merino, Ana
Hoogduijn, Martin J.
author_role author
author2 Luk, Franka
Korevaar, Sander S.
Bouzid, Rachid
Paz, Ana Helena da Rosa
Iglesias, Carmen López
Baan, Carla C.
Merino, Ana
Hoogduijn, Martin J.
author2_role author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Células mesenquimais estromais
Imunidade
Tecido adiposo
topic Células mesenquimais estromais
Imunidade
Tecido adiposo
Immunosuppression
Mesenchymal stem cells
dc.subject.eng.fl_str_mv Immunosuppression
Mesenchymal stem cells
description Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape. MP exhibited ATPase, nucleotidase and esterase activity, indicating they are enzymatically active. MP and MPγ did not physically interact with T cells and had no effect on CD4+ and CD8+ T cells proliferation. However, MP and MPγ selectively bound to monocytes and decreased the frequency of pro-inflammatory CD14+CD16+ monocytes by induction of selective apoptosis. MP and MPγ increased the percentage of CD90 positive monocytes, and MPγ but not MP increased the percentage of antiinflammatory PD-L1 monocytes. MPγ increased mRNA expression of PD-L1 in monocytes. These data demonstrate that MP have immunomodulatory properties and have potential as a novel cell-free therapy for treatment of immunological disorders.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-26T02:33:33Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/175085
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.nrb.pt_BR.fl_str_mv 001065696
identifier_str_mv 2045-2322
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url http://hdl.handle.net/10183/175085
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Scientific reports. London. Vol. 7 (Sep. 2017), 12100, 13 f.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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