Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs
The single photon emission microscope (SPEM) is an instrument developed to obtain high spatial resolution single photon emission computed tomography (SPECT) images of small structures inside the mouse brain. SPEM consists of two independent imaging devices, which combine a multipinhole collimator, a...
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Recursos: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/36913 |
| Acesso em linha: | http://dx.doi.org/10.1590/1414-431X20132764 http://repositorio.unifesp.br/handle/11600/36913 |
| Access Level: | acceso abierto |
| Palavra-chave: | Tomography Preclinical imaging Molecular imaging Mice |
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Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organsTomographyPreclinical imagingMolecular imagingMiceThe single photon emission microscope (SPEM) is an instrument developed to obtain high spatial resolution single photon emission computed tomography (SPECT) images of small structures inside the mouse brain. SPEM consists of two independent imaging devices, which combine a multipinhole collimator, a high-resolution, thallium-doped cesium iodide [CsI(Tl)] columnar scintillator, a demagnifying/intensifier tube, and an electron-multiplying charge-coupling device (CCD). Collimators have 300- and 450-mm diameter pinholes on tungsten slabs, in hexagonal arrays of 19 and 7 holes. Projection data are acquired in a photon-counting strategy, where CCD frames are stored at 50 frames per second, with a radius of rotation of 35 mm and magnification factor of one. the image reconstruction software tool is based on the maximum likelihood algorithm. Our aim was to evaluate the spatial resolution and sensitivity attainable with the seven-pinhole imaging device, together with the linearity for quantification on the tomographic images, and to test the instrument in obtaining tomographic images of different mouse organs. A spatial resolution better than 500 mm and a sensitivity of 21.6 counts?.s(-1).MBq(-1) were reached, as well as a correlation coefficient between activity and intensity better than 0.99, when imaging Tc-99m sources. Images of the thyroid, heart, lungs, and bones of mice were registered using Tc-99m-labeled radiopharmaceuticals in times appropriate for routine preclinical experimentation of <1 h per projection data set. Detailed experimental protocols and images of the aforementioned organs are shown. We plan to extend the instrument's field of view to fix larger animals and to combine data from both detectors to reduce the acquisition time or applied activity.Hosp Israelita Albert Einstein, Inst Cerebro, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psiquiatria, Lab Interdisciplinar Neurociencias Clin, São Paulo, BrazilGE Global Res, Schenectady, NY USAUniv Chicago, Dept Radiol, Chicago, IL 60637 USAUniv Illinois, Dept Nucl Plasma & Radiol Engn, Urbana, IL 61801 USAUniversidade Federal de São Paulo, Dept Psiquiatria, Lab Interdisciplinar Neurociencias Clin, São Paulo, BrazilWeb of ScienceAssociacao Beneficente Alzira Denise Hertzog da Silva (ABADHS)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2006/61333-3FAPESP: 2008/08902-5Assoc Bras Divulg CientificaHosp Israelita Albert EinsteinUniversidade Federal de São Paulo (UNIFESP)GE Global ResUniv ChicagoUniv Illinois2016-01-24T14:34:38Z2016-01-24T14:34:38Z2013-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion936-942application/pdfhttp://dx.doi.org/10.1590/1414-431X20132764Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 46, n. 11, p. 936-942, 2013.10.1590/1414-431X20132764S0100-879X2013001100936.pdf0100-879XS0100-879X2013001100936http://repositorio.unifesp.br/handle/11600/36913WOS:000328203500005ark:/48912/001300002gthkengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPMejia, JorgeReis, Marília Alves dos [UNIFESP]Miranda, Andrés C. C.Batista, Ilza Rosa [UNIFESP]Barboza, Marycel Rosa F.Shih, Ming ChiFu, GengChen, Chin TuMeng, Ling JianBressan, Rodrigo Affonseca [UNIFESP]Amaro, Edson2024-07-31T16:09:33Zoai:repositorio.unifesp.br:11600/36913Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T16:09:33Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| title |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| spellingShingle |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs Mejia, Jorge Tomography Preclinical imaging Molecular imaging Mice |
| title_short |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| title_full |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| title_fullStr |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| title_full_unstemmed |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| title_sort |
Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs |
| dc.creator.none.fl_str_mv |
Mejia, Jorge Reis, Marília Alves dos [UNIFESP] Miranda, Andrés C. C. Batista, Ilza Rosa [UNIFESP] Barboza, Marycel Rosa F. Shih, Ming Chi Fu, Geng Chen, Chin Tu Meng, Ling Jian Bressan, Rodrigo Affonseca [UNIFESP] Amaro, Edson |
| author |
Mejia, Jorge |
| author_facet |
Mejia, Jorge Reis, Marília Alves dos [UNIFESP] Miranda, Andrés C. C. Batista, Ilza Rosa [UNIFESP] Barboza, Marycel Rosa F. Shih, Ming Chi Fu, Geng Chen, Chin Tu Meng, Ling Jian Bressan, Rodrigo Affonseca [UNIFESP] Amaro, Edson |
| author_role |
author |
| author2 |
Reis, Marília Alves dos [UNIFESP] Miranda, Andrés C. C. Batista, Ilza Rosa [UNIFESP] Barboza, Marycel Rosa F. Shih, Ming Chi Fu, Geng Chen, Chin Tu Meng, Ling Jian Bressan, Rodrigo Affonseca [UNIFESP] Amaro, Edson |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Hosp Israelita Albert Einstein Universidade Federal de São Paulo (UNIFESP) GE Global Res Univ Chicago Univ Illinois |
| dc.subject.por.fl_str_mv |
Tomography Preclinical imaging Molecular imaging Mice |
| topic |
Tomography Preclinical imaging Molecular imaging Mice |
| description |
The single photon emission microscope (SPEM) is an instrument developed to obtain high spatial resolution single photon emission computed tomography (SPECT) images of small structures inside the mouse brain. SPEM consists of two independent imaging devices, which combine a multipinhole collimator, a high-resolution, thallium-doped cesium iodide [CsI(Tl)] columnar scintillator, a demagnifying/intensifier tube, and an electron-multiplying charge-coupling device (CCD). Collimators have 300- and 450-mm diameter pinholes on tungsten slabs, in hexagonal arrays of 19 and 7 holes. Projection data are acquired in a photon-counting strategy, where CCD frames are stored at 50 frames per second, with a radius of rotation of 35 mm and magnification factor of one. the image reconstruction software tool is based on the maximum likelihood algorithm. Our aim was to evaluate the spatial resolution and sensitivity attainable with the seven-pinhole imaging device, together with the linearity for quantification on the tomographic images, and to test the instrument in obtaining tomographic images of different mouse organs. A spatial resolution better than 500 mm and a sensitivity of 21.6 counts?.s(-1).MBq(-1) were reached, as well as a correlation coefficient between activity and intensity better than 0.99, when imaging Tc-99m sources. Images of the thyroid, heart, lungs, and bones of mice were registered using Tc-99m-labeled radiopharmaceuticals in times appropriate for routine preclinical experimentation of <1 h per projection data set. Detailed experimental protocols and images of the aforementioned organs are shown. We plan to extend the instrument's field of view to fix larger animals and to combine data from both detectors to reduce the acquisition time or applied activity. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-11-01 2016-01-24T14:34:38Z 2016-01-24T14:34:38Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X20132764 Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 46, n. 11, p. 936-942, 2013. 10.1590/1414-431X20132764 S0100-879X2013001100936.pdf 0100-879X S0100-879X2013001100936 http://repositorio.unifesp.br/handle/11600/36913 WOS:000328203500005 |
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ark:/48912/001300002gthk |
| url |
http://dx.doi.org/10.1590/1414-431X20132764 http://repositorio.unifesp.br/handle/11600/36913 |
| identifier_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 46, n. 11, p. 936-942, 2013. 10.1590/1414-431X20132764 S0100-879X2013001100936.pdf 0100-879X S0100-879X2013001100936 WOS:000328203500005 ark:/48912/001300002gthk |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Brazilian Journal of Medical and Biological Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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936-942 application/pdf |
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Assoc Bras Divulg Cientifica |
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Assoc Bras Divulg Cientifica |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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