Nanofibras biodegradáveis contendo furoato de mometasona: desenvolvimento, caracterização e avaliação da segurança em modelo experimental
Rhinosinusitis is a disease that affects a large part of the world population, causing a significant burden for society in health costs and loss of productivity. Chronic rhinosinusitis represents a significant health problem that affects 5 to 12% of the population. The treatment of choice is the use...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/46402 |
| Acceso en línea: | http://hdl.handle.net/1843/46402 |
| Access Level: | acceso abierto |
| Palabra clave: | Furoato de mometasona Copolímero dos ácidos glicólico e láctico Liberação sustentada Rinossinusite crônica Nanofibras Planejamento de experimentos |
| Sumario: | Rhinosinusitis is a disease that affects a large part of the world population, causing a significant burden for society in health costs and loss of productivity. Chronic rhinosinusitis represents a significant health problem that affects 5 to 12% of the population. The treatment of choice is the use of intranasal glucocorticoids, which have restricted access to the paranasal sinuses. Our new approach consists of the development of mometasone furoate biodegradable polymeric nanofibers. The new system, to treat chronic rhinosinusitis, provides a controlled release of glucocorticoids in the nasosinusal mucosa. This study aimed to develop biodegradable polymeric nanofibers and explore these fibers' safety in an in vivo rabbit model. The nanofibers development was optimized using the Response Surface Methodology and Design of Experiments to achieve the best polymer concentration and solvent ratio for the electrospinning process. The ideal system, determined by nanofiber's diameter responses, was analyzed by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, differential scanning calorimetry, assay, and drug delivery by a validated chromatographic method. The results showed that mometasone furoate is dispersed in the polymeric matrix, is stable, and showed sustained release kinetics in a bio-relevant nasal environment (Higuchi model kinetics). In vivo tests, the inflammation level of the animals' mucosa which received the mometasone furoate-loaded nanofiber was lower than those that received the nanofibers without the drug (α = 0.05). Histopathology analysis showed that the mometasone furoate-loaded nanofibers are safe when topically applied on the sinonasal mucosa, opening a new horizon in chronic rhinosinusitis treatment. |
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