Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis

Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Ri...

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Autores: Ramos, Tadeu Diniz, Silva, Johnatas Dutra, Martins, Alessandra Marcia da Fonseca, Pratti, Juliana Elena da Silveira, Cruz, Luan Firmino, Oliveira, Diogo Maciel, Santos, Julio Souza dos, Tenorio, João Ivo Nunes, Araujo, Almair Ferreira de, Lima, Celio Geraldo Freire de, Diaz, Bruno Lourenço, Crruz, Fernanda Ferreira, Rocco, Patricia Rieken Macedo, Guedes, Herbert Leonel de Matos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/45723
Acceso en línea:https://arca.fiocruz.br/handle/icict/45723
Access Level:acceso abierto
Palabra clave:Leishmaniose
Células Mesenquimais Estromais
Células mesenquimal derivadas do tecido adiposo
Antimoniato de meglumina
Células mesenquimais mesenquimais derivadas da medula óssea
Carga de parasitas
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dc.title.none.fl_str_mv Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
title Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
spellingShingle Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
Ramos, Tadeu Diniz
Leishmaniose
Células Mesenquimais Estromais
Células mesenquimal derivadas do tecido adiposo
Antimoniato de meglumina
Células mesenquimais mesenquimais derivadas da medula óssea
Carga de parasitas
title_short Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
title_full Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
title_fullStr Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
title_full_unstemmed Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
title_sort Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
dc.creator.none.fl_str_mv Ramos, Tadeu Diniz
Silva, Johnatas Dutra
Martins, Alessandra Marcia da Fonseca
Pratti, Juliana Elena da Silveira
Cruz, Luan Firmino
Oliveira, Diogo Maciel
Santos, Julio Souza dos
Tenorio, João Ivo Nunes
Araujo, Almair Ferreira de
Lima, Celio Geraldo Freire de
Diaz, Bruno Lourenço
Crruz, Fernanda Ferreira
Rocco, Patricia Rieken Macedo
Guedes, Herbert Leonel de Matos
author Ramos, Tadeu Diniz
author_facet Ramos, Tadeu Diniz
Silva, Johnatas Dutra
Martins, Alessandra Marcia da Fonseca
Pratti, Juliana Elena da Silveira
Cruz, Luan Firmino
Oliveira, Diogo Maciel
Santos, Julio Souza dos
Tenorio, João Ivo Nunes
Araujo, Almair Ferreira de
Lima, Celio Geraldo Freire de
Diaz, Bruno Lourenço
Crruz, Fernanda Ferreira
Rocco, Patricia Rieken Macedo
Guedes, Herbert Leonel de Matos
author_role author
author2 Silva, Johnatas Dutra
Martins, Alessandra Marcia da Fonseca
Pratti, Juliana Elena da Silveira
Cruz, Luan Firmino
Oliveira, Diogo Maciel
Santos, Julio Souza dos
Tenorio, João Ivo Nunes
Araujo, Almair Ferreira de
Lima, Celio Geraldo Freire de
Diaz, Bruno Lourenço
Crruz, Fernanda Ferreira
Rocco, Patricia Rieken Macedo
Guedes, Herbert Leonel de Matos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Leishmaniose
Células Mesenquimais Estromais
Células mesenquimal derivadas do tecido adiposo
Antimoniato de meglumina
Células mesenquimais mesenquimais derivadas da medula óssea
Carga de parasitas
topic Leishmaniose
Células Mesenquimais Estromais
Células mesenquimal derivadas do tecido adiposo
Antimoniato de meglumina
Células mesenquimais mesenquimais derivadas da medula óssea
Carga de parasitas
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-16T14:05:21Z
dc.date.available.fl_str_mv 2021-01-16T14:05:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/45723
dc.identifier.issn.none.fl_str_mv 2637-9589
dc.identifier.doi.none.fl_str_mv 10.1186/s13287-020-01889-z
identifier_str_mv RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020.
2637-9589
10.1186/s13287-020-01889-z
url https://arca.fiocruz.br/handle/icict/45723
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2021-01-16T14:05:21Z2021-01-16T14:05:21Z2020RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020.2637-9589https://arca.fiocruz.br/handle/icict/4572310.1186/s13287-020-01889-zengBMCLeishmanioseCélulas Mesenquimais EstromaisCélulas mesenquimal derivadas do tecido adiposoAntimoniato de megluminaCélulas mesenquimais mesenquimais derivadas da medula ósseaCarga de parasitasCombined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Medicina Regeneratica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus Duque de Caxias Professor Geraldo Cidade. Duque de Caxias, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Background: Leishmaniasis is a neglected disease caused by Leishmania spp. One of its characteristics is an imbalance of host immune responses to foster parasite survival. In this setting, mesenchymal stromal cells (MSCs) may be a viable therapeutic alternative, given their well-established immunomodulatory potential. In this study, we compared the effects of therapy with bone marrow (BM)- and adipose tissue (AD)-derived MSCs in leishmaniasis caused by Leishmania amazonensis in C57BL/6 mice. After determining the most effective MSC source, we then combined these cells with meglumine antimoniate (a pentavalent antimonial commonly used for the treatment of leishmaniasis) to treat the infected mice. Methods: In vitro, co-culture of AD-MSCs and BM-MSCs with Leishmania amazonensis-infected macrophages was performed to understand the influence of both MSC sources in infected cells. In vivo, infected C57BL/6 mice were treated with phosphate-buffered saline (PBS), AD-MSCs and BM-MSCs, and then meglumine antimoniate was combined with MSCs from the most effective source.Results: In vitro, co-culture of Leishmania amazonensis-infected macrophages with BM-MSCs, compared to ADMSCs, led to a higher parasite load and lower production of nitric oxide. Fibroblasts grown in conditioned medium from co-cultures with AD-MSCs promoted faster wound healing. Despite a non-significant difference in the production of vascular endothelial growth factor, we observed higher production of tumor necrosis factor-α and interleukin (IL)-10 in the co-culture with AD-MSCs. In vivo, treatment of infected mice with BM-MSCs did not lead to disease control; however, the use of AD-MSCs was associated with partial control of lesion development, without significant differences in the parasite load. AD-MSCs combined with meglumine antimoniate reduced lesion size and parasite load when compared to PBS and AD-MSC groups. At the infection site, we detected a small production of IL-10, but we were unable to detect production of either IL-4 or interferon-γ, indicating resolution of infection without effect on the percentage of regulatory T cells. Conclusion: Combination treatment of cutaneous leishmaniasis with AD-MSCs and meglumine antimoniate may be a viable alternative.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZRamos, Tadeu DinizSilva, Johnatas DutraMartins, Alessandra Marcia da FonsecaPratti, Juliana Elena da SilveiraCruz, Luan FirminoOliveira, Diogo MacielSantos, Julio Souza dosTenorio, João Ivo NunesAraujo, Almair Ferreira deLima, Celio Geraldo Freire deDiaz, Bruno LourençoCrruz, Fernanda FerreiraRocco, Patricia Rieken MacedoGuedes, Herbert Leonel de MatosLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/b86d7c95-9811-44fd-93a5-9eb3dfecaec3/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALHerbetLM_Guedes_etal_IOC_2020.pdfHerbetLM_Guedes_etal_IOC_2020.pdfapplication/pdf1535937https://arca.fiocruz.br/bitstreams/cffcc210-37bd-4c08-bf18-afb00b67d5f0/download4a0a23eb981d92fbb0552f35aab8b4f3MD52trueAnonymousREADTEXTHerbetLM_Guedes_etal_IOC_2020.pdf.txtHerbetLM_Guedes_etal_IOC_2020.pdf.txtExtracted texttext/plain76818https://arca.fiocruz.br/bitstreams/5735a071-7daa-4108-ac3b-f531844f8151/download3bac71f40068538a8964dba8d82a83a1MD515falseAnonymousREADTHUMBNAILHerbetLM_Guedes_etal_IOC_2020.pdf.jpgHerbetLM_Guedes_etal_IOC_2020.pdf.jpgGenerated Thumbnailimage/jpeg37509https://arca.fiocruz.br/bitstreams/46a0f0e7-03d5-4d9a-807e-4787775b0d45/downloadbddbe9b2f747f5df19bb303a7fcdaba7MD516falseAnonymousREADicict/457232025-12-16 06:31:04.226open.accessoai:arca.fiocruz.br:icict/45723https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-16T09:31:04Repositório Institucional da FIOCRUZ (ARCA) - 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