Prebiótico e probiótico para leitões experimentalmente desafiados com Escherichia coli K88+
With the objective of evaluating the properties of the prebiotic manno-oligosaccharides and probiotic Bacillus subtilis in piglets experimentally infected with E. coli (1010 Colony Forming Units), a study was conducted at the Experimental Center of Swine of the UFLA, in Lavras, MG. A total of 75 pig...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Institución: | Universidade Federal de Lavras (UFLA) |
| Repositorio: | Repositório Institucional da UFLA |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufla.br:1/2003 |
| Acceso en línea: | https://repositorio.ufla.br/handle/1/2003 |
| Access Level: | acceso abierto |
| Palabra clave: | CNPQ_NÃO_INFORMADO Mucosa intestinal Promotor de crescimento Saúde intestinal Suínos Vilosidade Growth promoter Gut health Intestinal mucosae Pigs Villi |
| Sumario: | With the objective of evaluating the properties of the prebiotic manno-oligosaccharides and probiotic Bacillus subtilis in piglets experimentally infected with E. coli (1010 Colony Forming Units), a study was conducted at the Experimental Center of Swine of the UFLA, in Lavras, MG. A total of 75 piglets (females and castrated males), weaned at 28 days old, average initial weight of 8.44 kg ± 2.19 kg, was used. Performance parameters, microbial population, intestinal morphology, immune function, incidence of diarrhea, pH and short-chain fatty acid (SCFA) concentration in the cecum and colon were studied. The experimental period lasted 35 days, with randomized block design, consisting of the following treatments: NCO - halquinol without immunological challenge (negative control); PCO - halquinol with immunological challenge (positive control); MO - prebiotic with immunological challenge; BS - probiotic with immunological challenge; MOBS - probiotic + prebiotic immunological challenge; with a total of five treatments and five replications. The animals were standardized according to weight at weaning and housed in groups of three pigs per pen representing the plot. The performance variables were not affected by the diet (P> 0.5). However, the use of additives provided greater length of the small intestine (P <0.05), while animals fed only the prebiotic presented lower relative weights of the small intestine (P <0.05). Population of total E. coli in the ileum of animals not challenged was lower (P <0.05) when compared to other treatments; pH values of the cecum and colon were not influenced by the diet (P> 0.05). However, the concentrations of propionic acid were lower (P <0.05) in the cecum of animals fed either probiotic or prebiotic. Histological studies showed that animals fed only probiotics had a lower height of villi in the ileum (P <0.05). The villous:crypt ratio was higher in the ileum of the control group. Total levels of IgA, IgG and IgM were similar (P> 0.05) in all treatments. No cases of diarrhea during the experimental period were found. In general, the tested additives alter both the intestinal morphology and VFA production, their being as effective as the use of antibiotic in the other parameters evaluated. |
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