Blood cell attachment to root surfaces treated with EDTA gel.

Root debridement generates a smear layer which contains microorganisms and toxins that could interfere in periodontal healing. For this reason, different substances have been used to remove it and to expose collagen fibers at the tooth surface. Blood element adhesion to demineralized roots and clot...

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Detalles Bibliográficos
Autores: Leite, Fábio Renato Manzolli, Moreira, Camila Sala, Theodoro, Letícia Helena [UNESP], Sampaio, José Eduardo Cezar [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/68184
Acceso en línea:http://dx.doi.org/10.1590/S1806-83242005000200003
http://hdl.handle.net/11449/68184
Access Level:acceso abierto
Palabra clave:chelating agent
edetic acid
adsorption
cell adhesion
comparative study
dental surgery
dentin
drug effect
endodontics
gel
human
methodology
nonparametric test
physiology
preventive dentistry
tooth root
ultrastructure
Adsorption
Cell Adhesion
Chelating Agents
Dental Prophylaxis
Dentin
Edetic Acid
Gels
Humans
Root Canal Preparation
Smear Layer
Statistics, Nonparametric
Tooth Root
Descripción
Sumario:Root debridement generates a smear layer which contains microorganisms and toxins that could interfere in periodontal healing. For this reason, different substances have been used to remove it and to expose collagen fibers at the tooth surface. Blood element adhesion to demineralized roots and clot stabilization by collagen fibers are extremely important for the success of periodontal surgery. The aim of this study was to evaluate the different patterns of blood element adsorption and adhesion to root surfaces only irrigated with distilled water and after application of a manipulated or an industrialized EDTA gel. Thirty samples were planed, equally divided into three groups and treated with distilled water (control), a manipulated EDTA gel or an industrialized one. Immediately after, samples were exposed to fresh blood and prepared for scanning electron microscopy. Untreated planed dentin presented the best results with blood cells entrapped in a thick web of fibrin. In the manipulated EDTA group, the web of fibrin was thick with sparse blood elements. The worst result was seen with the industrialized EDTA group, in which no blood elements could be seen. Statistical difference was obtained between control and industrialized EDTA groups. Surfaces only irrigated presented the most organized fibrin network and cell entrapment.