Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A
Monastrol and its analog oxomonastrol differ by replacement of the sulfur atom present in monastrol to an oxygen atom in oxomonastrol. Monastrol inhibits the mitotic kinesin family member 11 (EG5), which has been studied for its potential use in cancer therapy. The aim of this study was to investiga...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/70470 |
| Acceso en línea: | https://doi.org/10.21873/anticanres.11434 http://hdl.handle.net/1843/70470 https://orcid.org/0000-0003-2344-5590 https://orcid.org/0000-0002-7550-8509 https://orcid.org/0000-0002-0465-9932 https://orcid.org/0000-0002-9857-8681 https://orcid.org/0000-0001-5268-6508 |
| Access Level: | acceso abierto |
| Palabra clave: | Monastrol Oxo-monastrol Human hepatoma cell line Anticancer activity Morfologia Química Agentes antineoplásicos Apoptose Testes biológicos Microscopia de fluorescência Câncer - Quimioterapia |
| id |
BR_7b176190872e65ac75a962b4675c5b13 |
|---|---|
| oai_identifier_str |
oai:repositorio.ufmg.br:1843/70470 |
| network_acronym_str |
BR |
| network_name_str |
Brasil |
| repository_id_str |
|
| spelling |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3AMonastrolOxo-monastrolHuman hepatoma cell lineAnticancer activityMorfologiaQuímicaAgentes antineoplásicosApoptoseTestes biológicosMicroscopia de fluorescênciaCâncer - QuimioterapiaMonastrol and its analog oxomonastrol differ by replacement of the sulfur atom present in monastrol to an oxygen atom in oxomonastrol. Monastrol inhibits the mitotic kinesin family member 11 (EG5), which has been studied for its potential use in cancer therapy. The aim of this study was to investigate the effect of monastrol and oxomonastrol on HepG2/C3A cells. Our results showed that monastrol induced DNA damage, reduced cell proliferation, and up-regulated the cytochrome P450 family 1 subfamily A member 1 (CYP1A1) mRNA levels. However, oxomonastrol was cytotoxic only at the highest concentrations used, without reducing cell proliferation and viability. Moreover, no genotoxic damage or alteration of levels of mRNA were found. Our results suggest that monastrol has greater antiproliferative activity compared to oxomonastrol, and this effect is probably related to the DNA damage induced by monastrol and its possible bioactivation demonstrated by the increase in CYP1A1 mRNA expression. Moreover, these effects appear to be related to the presence of the sulfur atom in its structure.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaUniversidade Federal de Minas GeraisBrasilICX - DEPARTAMENTO DE QUÍMICAUFMG2024-07-12T19:55:45Z2024-07-12T19:55:45Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdfhttps://doi.org/10.21873/anticanres.114341791-7530http://hdl.handle.net/1843/70470https://orcid.org/0000-0003-2344-5590https://orcid.org/0000-0002-7550-8509https://orcid.org/0000-0002-0465-9932https://orcid.org/0000-0002-9857-8681https://orcid.org/0000-0001-5268-6508engAnticancer Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLilian Areal MarquesSimone Cristine SemprebonDaniele SartoriÂngelo de FátimaLúcia Regina RibeiroMário Sérgio Mantovani2024-07-12T19:55:45Zoai:repositorio.ufmg.br:1843/70470Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2024-07-12T19:55:45Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.none.fl_str_mv |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| title |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| spellingShingle |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A Lilian Areal Marques Monastrol Oxo-monastrol Human hepatoma cell line Anticancer activity Morfologia Química Agentes antineoplásicos Apoptose Testes biológicos Microscopia de fluorescência Câncer - Quimioterapia |
| title_short |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| title_full |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| title_fullStr |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| title_full_unstemmed |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| title_sort |
Comparison of the effects of monastrol and oxomonastrol on human hepatoma cell line HepG2/C3A |
| dc.creator.none.fl_str_mv |
Lilian Areal Marques Simone Cristine Semprebon Daniele Sartori Ângelo de Fátima Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author |
Lilian Areal Marques |
| author_facet |
Lilian Areal Marques Simone Cristine Semprebon Daniele Sartori Ângelo de Fátima Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author_role |
author |
| author2 |
Simone Cristine Semprebon Daniele Sartori Ângelo de Fátima Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author2_role |
author author author author author |
| dc.subject.por.fl_str_mv |
Monastrol Oxo-monastrol Human hepatoma cell line Anticancer activity Morfologia Química Agentes antineoplásicos Apoptose Testes biológicos Microscopia de fluorescência Câncer - Quimioterapia |
| topic |
Monastrol Oxo-monastrol Human hepatoma cell line Anticancer activity Morfologia Química Agentes antineoplásicos Apoptose Testes biológicos Microscopia de fluorescência Câncer - Quimioterapia |
| description |
Monastrol and its analog oxomonastrol differ by replacement of the sulfur atom present in monastrol to an oxygen atom in oxomonastrol. Monastrol inhibits the mitotic kinesin family member 11 (EG5), which has been studied for its potential use in cancer therapy. The aim of this study was to investigate the effect of monastrol and oxomonastrol on HepG2/C3A cells. Our results showed that monastrol induced DNA damage, reduced cell proliferation, and up-regulated the cytochrome P450 family 1 subfamily A member 1 (CYP1A1) mRNA levels. However, oxomonastrol was cytotoxic only at the highest concentrations used, without reducing cell proliferation and viability. Moreover, no genotoxic damage or alteration of levels of mRNA were found. Our results suggest that monastrol has greater antiproliferative activity compared to oxomonastrol, and this effect is probably related to the DNA damage induced by monastrol and its possible bioactivation demonstrated by the increase in CYP1A1 mRNA expression. Moreover, these effects appear to be related to the presence of the sulfur atom in its structure. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2024-07-12T19:55:45Z 2024-07-12T19:55:45Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.21873/anticanres.11434 1791-7530 http://hdl.handle.net/1843/70470 https://orcid.org/0000-0003-2344-5590 https://orcid.org/0000-0002-7550-8509 https://orcid.org/0000-0002-0465-9932 https://orcid.org/0000-0002-9857-8681 https://orcid.org/0000-0001-5268-6508 |
| url |
https://doi.org/10.21873/anticanres.11434 http://hdl.handle.net/1843/70470 https://orcid.org/0000-0003-2344-5590 https://orcid.org/0000-0002-7550-8509 https://orcid.org/0000-0002-0465-9932 https://orcid.org/0000-0002-9857-8681 https://orcid.org/0000-0001-5268-6508 |
| identifier_str_mv |
1791-7530 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Anticancer Research |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil ICX - DEPARTAMENTO DE QUÍMICA UFMG |
| publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil ICX - DEPARTAMENTO DE QUÍMICA UFMG |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| instname_str |
Universidade Federal de Minas Gerais (UFMG) |
| instacron_str |
UFMG |
| institution |
UFMG |
| reponame_str |
Repositório Institucional da UFMG |
| collection |
Repositório Institucional da UFMG |
| repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
| repository.mail.fl_str_mv |
repositorio@ufmg.br |
| _version_ |
1853663321244303360 |
| score |
15,301603 |