Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).

The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using...

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Autores: Carvalho, Edirsana Maria Ribeiro, Menezes, Francisca Gleire Rodrigues de, Santos, Daniel Rodrigues dos, Souza, Oscarina Viana de, Hofer, Ernesto, Vieira, Regine Helena silva dos Fernandes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:inglés
OAI Identifier:oai:repositorio.ufc.br:riufc/81250
Acceso en línea:http://repositorio.ufc.br/handle/riufc/81250
Access Level:acceso abierto
Palabra clave:Aquicultura
Antibiótico
Integron
Aquaculture
Antibiotic
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network_name_str Brasil
repository_id_str
dc.title.pt_BR.fl_str_mv Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
title Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
spellingShingle Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
Carvalho, Edirsana Maria Ribeiro
Aquicultura
Antibiótico
Integron
Aquaculture
Antibiotic
Integron
title_short Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
title_full Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
title_fullStr Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
title_full_unstemmed Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
title_sort Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
dc.creator.none.fl_str_mv Carvalho, Edirsana Maria Ribeiro
Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena silva dos Fernandes
author Carvalho, Edirsana Maria Ribeiro
author_facet Carvalho, Edirsana Maria Ribeiro
Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena silva dos Fernandes
author_role author
author2 Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena silva dos Fernandes
author2_role author
author
author
author
author
dc.subject.ptbr.pt_BR.fl_str_mv Aquicultura
Antibiótico
Integron
topic Aquicultura
Antibiótico
Integron
Aquaculture
Antibiotic
Integron
dc.subject.en.pt_BR.fl_str_mv Aquaculture
Antibiotic
Integron
description The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2025-06-11T12:42:58Z
dc.date.available.fl_str_mv 2025-06-11T12:42:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Edirsana Maria Ribeiro; MENEZES, Francisca Gleire Rodrigues des; SANTOS, Daniel Rodrigues dos; SOUZA, Oscarina Viana de; HOFER, Ernesto; VIEIRA, Regine Helena Silva dos Fernandes. Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Revista Científica Semana Acadêmica, v. 10, p. 1-16, 2022. Disponível em: https://semanaacademica.org.br/artigo/integrons-and-gene-cassettes-encoding-antimicrobial-resistance-vibrios-isolated-shrimp. Acesso em: 11 jun 2025.
dc.identifier.uri.fl_str_mv http://repositorio.ufc.br/handle/riufc/81250
dc.identifier.doi.none.fl_str_mv 2236-6717
identifier_str_mv CARVALHO, Edirsana Maria Ribeiro; MENEZES, Francisca Gleire Rodrigues des; SANTOS, Daniel Rodrigues dos; SOUZA, Oscarina Viana de; HOFER, Ernesto; VIEIRA, Regine Helena Silva dos Fernandes. Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Revista Científica Semana Acadêmica, v. 10, p. 1-16, 2022. Disponível em: https://semanaacademica.org.br/artigo/integrons-and-gene-cassettes-encoding-antimicrobial-resistance-vibrios-isolated-shrimp. Acesso em: 11 jun 2025.
2236-6717
url http://repositorio.ufc.br/handle/riufc/81250
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Revista Científica Semana Acadêmica
publisher.none.fl_str_mv Revista Científica Semana Acadêmica
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/81250/1/2022_art_emrcarvalho.pdf
http://repositorio.ufc.br/bitstream/riufc/81250/2/license.txt
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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spelling 2025-06-11T12:42:58Z2025-06-11T12:42:58Z2022CARVALHO, Edirsana Maria Ribeiro; MENEZES, Francisca Gleire Rodrigues des; SANTOS, Daniel Rodrigues dos; SOUZA, Oscarina Viana de; HOFER, Ernesto; VIEIRA, Regine Helena Silva dos Fernandes. Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Revista Científica Semana Acadêmica, v. 10, p. 1-16, 2022. Disponível em: https://semanaacademica.org.br/artigo/integrons-and-gene-cassettes-encoding-antimicrobial-resistance-vibrios-isolated-shrimp. Acesso em: 11 jun 2025.http://repositorio.ufc.br/handle/riufc/812502236-6717The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment.O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacE∆1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão.Revista Científica Semana AcadêmicaIntegrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAquiculturaAntibióticoIntegronAquacultureAntibioticIntegroninfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCCarvalho, Edirsana Maria RibeiroMenezes, Francisca Gleire Rodrigues deSantos, Daniel Rodrigues dosSouza, Oscarina Viana deHofer, ErnestoVieira, Regine Helena silva dos Fernandeshttp://lattes.cnpq.br/0653219789872234ORIGINAL2022_art_emrcarvalho.pdf2022_art_emrcarvalho.pdfapplication/pdf808675http://repositorio.ufc.br/bitstream/riufc/81250/1/2022_art_emrcarvalho.pdf89e57e0af134b8d20aa8e6e379b42716MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/81250/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufc/812502025-06-17 10:58:44.773oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2025-06-17T13:58:44Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
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