Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI).
The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade Federal do Ceará (UFC) |
| Repositorio: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufc.br:riufc/81250 |
| Acceso en línea: | http://repositorio.ufc.br/handle/riufc/81250 |
| Access Level: | acceso abierto |
| Palabra clave: | Aquicultura Antibiótico Integron Aquaculture Antibiotic |
| Sumario: | The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment. |
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