Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico
We measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical test...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1998 |
| País: | Brasil |
| Institución: | Instituto Lauro de Souza Lima (ILSL) |
| Repositorio: | Hansenologia Internationalis (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/36461 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461 |
| Access Level: | acceso abierto |
| Palabra clave: | Hanseníase NADH-redutase Diaforase NADH-metemoglobina-redutase Leprosy NADH- reductase Diaphorase NADH-methemoglobin-reductase |
| Sumario: | We measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical tests, except for sulphone measure, were applied to blood samples from 72 healthy individuals who did not ingest any oxidant drug. Significant statistical differences regarding the values in erythrocytes, methemoglobin, reticulocytes and activity of NADH- reductase in the erythrocytes ghosts were found among these two groups. The mean enzyme activity in the hemolysate of the leprosy patients and thehealthy individuals has not differed significantly when expressed in UI/g.Hemoglobin/l. The enzyme activity in erythrocytes ghosts from leprosy patients was significantly smaller then that in erythrocytes ghosts from healthy individuals. The mean level of methemoglobin in the leprosy patients was higher than in the control group, probably due to oxidizer action of sulphone. Tends in view we have found smaller levels of enzymatic activity in the erythrocytes membranes of the leprosy patients and coincident levels in the hemolysate compared to controls, we can affirm that probably this is due to an enzymatic displacement from the cellular membrane to the cytoplasm, with the objective of maintaining in the erythrocyte a constant hemoglobinic balance, in spite of the sulphone oxidizer action. |
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