Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.

Oldoni, Tatiane Luiza Cadorin; Melo, Priscilla Siqueira; Massarioli, Adna Prado; Moreno, Ivani Aparecida Marchetto; Bezerra, Rosangela Maria Neves; Rosalen, Pedro Luiz; Silva, Gil Valdo José da; Nascimento, Andréa Mendes do; Alencar, Severino Matias de

Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.

Purification and bioassay-guided fractionation were employed to isolate proanthocyanidins with antioxidant activity from peanut skin (Arachis hypogaea Runner 886). The crude extract was prepared with acetone (60% v/v) and purified using chromatographic methods, including a semipreparative HPLC techn...

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Autores:	Oldoni, Tatiane Luiza Cadorin, Melo, Priscilla Siqueira, Massarioli, Adna Prado, Moreno, Ivani Aparecida Marchetto, Bezerra, Rosangela Maria Neves, Rosalen, Pedro Luiz, Silva, Gil Valdo José da, Nascimento, Andréa Mendes do, Alencar, Severino Matias de
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2016
País:	Brasil
Institución:	Universidade Federal de Ouro Preto (UFOP)
Repositorio:	Repositório Institucional da UFOP
Idioma:	inglés
OAI Identifier:	oai:repositorio.ufop.br:123456789/6678
Acceso en línea:	http://www.repositorio.ufop.br/handle/123456789/6678 https://doi.org/10.1016/j.foodchem.2015.07.004
Access Level:	acceso abierto
Palabra clave:	Purification Chromatography Antioxidant activity Phenolic compounds

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spelling	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.PurificationChromatographyAntioxidant activityPhenolic compoundsPurification and bioassay-guided fractionation were employed to isolate proanthocyanidins with antioxidant activity from peanut skin (Arachis hypogaea Runner 886). The crude extract was prepared with acetone (60% v/v) and purified using chromatographic methods, including a semipreparative HPLC technique. As a result, two proanthocyanidins were isolated and identified using NMR, epicatechin-(2 b?O?7, 4 b?8)-catechin (proanthocyanidin A1) and epicatechin-(b?2 O?7, 4 b?8)-epicatechin (proanthocyanidin A2). Despite the structural similarity, differences were observed in their antioxidant activity. Proanthocyanidin A1 proved to be more active, with EC50 value for DPPH radical scavenging of 18.25 lg/mL and reduction of Fe3+–TPTZ complex of 7.59 mmol/g, higher than that of synthetic antioxidant BHT. This compound evaluated by ABTS+_ was similar to that of natural quercetin. Therefore, peanut skin is an important source of bioactive compounds that may be used as a mild antioxidant for food preservation.2016-07-29T16:51:53Z2016-07-29T16:51:53Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLDONI,T. L. C. et al. Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Food Chemistry, v. 192, p. 306-312, 2016. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0308814615010092>. Acesso em: 16 jun. 2016.0308-8146http://www.repositorio.ufop.br/handle/123456789/6678https://doi.org/10.1016/j.foodchem.2015.07.004ark:/61566/0013000001w8rO periódico Food Chemistry concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3898810379758.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPOldoni, Tatiane Luiza CadorinMelo, Priscilla SiqueiraMassarioli, Adna PradoMoreno, Ivani Aparecida MarchettoBezerra, Rosangela Maria NevesRosalen, Pedro LuizSilva, Gil Valdo José daNascimento, Andréa Mendes doAlencar, Severino Matias de2025-02-09T01:04:36Zoai:repositorio.ufop.br:123456789/6678Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332025-02-09T01:04:36Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
title	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
spellingShingle	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Oldoni, Tatiane Luiza Cadorin Purification Chromatography Antioxidant activity Phenolic compounds
title_short	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
title_full	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
title_fullStr	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
title_full_unstemmed	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
title_sort	Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.
dc.creator.none.fl_str_mv	Oldoni, Tatiane Luiza Cadorin Melo, Priscilla Siqueira Massarioli, Adna Prado Moreno, Ivani Aparecida Marchetto Bezerra, Rosangela Maria Neves Rosalen, Pedro Luiz Silva, Gil Valdo José da Nascimento, Andréa Mendes do Alencar, Severino Matias de
author	Oldoni, Tatiane Luiza Cadorin
author_facet	Oldoni, Tatiane Luiza Cadorin Melo, Priscilla Siqueira Massarioli, Adna Prado Moreno, Ivani Aparecida Marchetto Bezerra, Rosangela Maria Neves Rosalen, Pedro Luiz Silva, Gil Valdo José da Nascimento, Andréa Mendes do Alencar, Severino Matias de
author_role	author
author2	Melo, Priscilla Siqueira Massarioli, Adna Prado Moreno, Ivani Aparecida Marchetto Bezerra, Rosangela Maria Neves Rosalen, Pedro Luiz Silva, Gil Valdo José da Nascimento, Andréa Mendes do Alencar, Severino Matias de
author2_role	author author author author author author author author
dc.subject.por.fl_str_mv	Purification Chromatography Antioxidant activity Phenolic compounds
topic	Purification Chromatography Antioxidant activity Phenolic compounds
description	Purification and bioassay-guided fractionation were employed to isolate proanthocyanidins with antioxidant activity from peanut skin (Arachis hypogaea Runner 886). The crude extract was prepared with acetone (60% v/v) and purified using chromatographic methods, including a semipreparative HPLC technique. As a result, two proanthocyanidins were isolated and identified using NMR, epicatechin-(2 b?O?7, 4 b?8)-catechin (proanthocyanidin A1) and epicatechin-(b?2 O?7, 4 b?8)-epicatechin (proanthocyanidin A2). Despite the structural similarity, differences were observed in their antioxidant activity. Proanthocyanidin A1 proved to be more active, with EC50 value for DPPH radical scavenging of 18.25 lg/mL and reduction of Fe3+–TPTZ complex of 7.59 mmol/g, higher than that of synthetic antioxidant BHT. This compound evaluated by ABTS+_ was similar to that of natural quercetin. Therefore, peanut skin is an important source of bioactive compounds that may be used as a mild antioxidant for food preservation.
publishDate	2016
dc.date.none.fl_str_mv	2016-07-29T16:51:53Z 2016-07-29T16:51:53Z 2016
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	OLDONI,T. L. C. et al. Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Food Chemistry, v. 192, p. 306-312, 2016. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0308814615010092>. Acesso em: 16 jun. 2016. 0308-8146 http://www.repositorio.ufop.br/handle/123456789/6678 https://doi.org/10.1016/j.foodchem.2015.07.004
dc.identifier.dark.fl_str_mv	ark:/61566/0013000001w8r
identifier_str_mv	OLDONI,T. L. C. et al. Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Food Chemistry, v. 192, p. 306-312, 2016. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0308814615010092>. Acesso em: 16 jun. 2016. 0308-8146 ark:/61566/0013000001w8r
url	http://www.repositorio.ufop.br/handle/123456789/6678 https://doi.org/10.1016/j.foodchem.2015.07.004
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP
instname_str	Universidade Federal de Ouro Preto (UFOP)
instacron_str	UFOP
institution	UFOP
reponame_str	Repositório Institucional da UFOP
collection	Repositório Institucional da UFOP
repository.name.fl_str_mv	Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv	repositorio@ufop.edu.br
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score	15,301603

Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.

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