The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage
The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sop...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2004 |
| Country: | Brasil |
| Institution: | Universidade Estadual Paulista (UNESP) |
| Repository: | Repositório Institucional da UNESP |
| Language: | English |
| OAI Identifier: | oai:repositorio.unesp.br:11449/3926 |
| Online Access: | http://dx.doi.org/10.1002/bmb.2004.494032010311 http://hdl.handle.net/11449/3926 |
| Access Level: | Open access |
| Keyword: | zymogen trypsin chymotrypsin enteropeptidase enzyme regulation proteolytic cleavage |
| Summary: | The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly switched on by specific proteolytic cleavage. |
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