Avaliação microbiológica in vivo do efeito do preparo biomecânico dos canais radiculares utilizando diferentes soluções irrigadoras

The aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following sol...

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Bibliographic Details
Authors: Tanomaru Filho, Mário, Yamashita, José Carlos, Leonardo, Mario Roberto, Silva, Léa Assed Bezerra da, Tanomaru, Juliane Maria Guerreiro, Ito, Izabel Yoko
Format: article
Status:Published version
Publication Date:2006
Country:Brasil
Institution:Universidade de São Paulo (USP)
Repository:Journal of applied oral science (Online)
Language:English
OAI Identifier:oai:revistas.usp.br:article/3352
Online Access:https://www.revistas.usp.br/jaos/article/view/3352
Access Level:Open access
Keyword:Endodontia
Microbiologia
Hipoclorito de sódio
Clorexidina
Endodontics
Microbiology
Sodium hypochlorite
Chlorhexidine
Description
Summary:The aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: Group 1) 2.5% sodium hypochlorite (NaOCl); Group 2) 2% chlorhexidine (CHX); Group 3) saline solution and Group 4) control group with no biomechanical preparation. The microbiological evaluation of the root canals was performed by counting the colony forming units (CFUs) using different culture mediums. Two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. The culture plates were incubated in aerobic, anaerobic and microaerophilic environment. Statistical evaluation was carried out using analysis of variance, Tukey and Student tests. The results demonstrated that there was reduction in the number of microorganisms in the NaOCl and CHX groups (p