Laboratory identification of pertussis in the western region of São Paulo State, Brazil

The aim of this study was to confirm the suspected cases of pertussis in the Western region of the Sao Paulo State from 2010 to 2015. The samples were cultured in the Instituto Adolfo Lutz - Regional Laboratory of Presidente Prudente-SP, and the qPCR was performed at the National Reference Laborator...

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Detalles Bibliográficos
Autores: Gushiken, Carolina Yumi, Saeki, Erika Kushikawa, Martins, Luciano Moura, Pereira, Juliana Cristina, Leite, Daniela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/34180
Acceso en línea:https://periodicos.saude.sp.gov.br/RIAL/article/view/34180
Access Level:acceso abierto
Palabra clave:Bordetella pertussis
laboratory diagnosis
Real-time PCR
pertussis
diagnóstico laboratorial
PCR em tempo real
coqueluche
Descripción
Sumario:The aim of this study was to confirm the suspected cases of pertussis in the Western region of the Sao Paulo State from 2010 to 2015. The samples were cultured in the Instituto Adolfo Lutz - Regional Laboratory of Presidente Prudente-SP, and the qPCR was performed at the National Reference Laboratory for Pertussis – Central Instituto Adolfo Lutz, São Paulo-SP. In this period, 189 samples were received, being 29 (15.3%) confirmed by the laboratory criteria (culture and/or qPCR). The most affected group was the children less than six months old (82.8%), not vaccinated or with the incomplete vaccination. Most likely, these results only represent a fraction of the actual number of pertussis cases occurring in Brazil. The continuous disease monitoring and the prevalence data by age group are fundamental to improve the immunization strategies as a way to control this important re-emerging disease.