Variações metodológicas na criopreservação de sêmen sexado de bovinos
The objective of this study was to evaluate the effects of 3 different cooling protocols for freezing bovine sexed semen by flow cytometry. It was used two ejaculates of 10 bulls, after collection of semen that was processed for separation of X and Y sperm. After separation by the cytometer, the sam...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/141279 |
| Acceso en línea: | http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/81 http://hdl.handle.net/11449/141279 |
| Access Level: | acceso abierto |
| Palabra clave: | Sexed semen Bovine Refrigeration Glycerol Cryopreservation Sêmen sexado Vacuno De refrigeración Glicerol Congelación Bovino Refrigeração Congelação |
| Sumario: | The objective of this study was to evaluate the effects of 3 different cooling protocols for freezing bovine sexed semen by flow cytometry. It was used two ejaculates of 10 bulls, after collection of semen that was processed for separation of X and Y sperm. After separation by the cytometer, the samples were divided into 3 groups: control (C), the sperm were placed in medium without glycerol and stored at 5°C/90 minutes, group 1 (G1), the sperm were placed in medium without glycerol and stored at 18ºC/90 minutes, group 2 (G2), the sperm were placed in medium with glycerol and stored at 5°C/90 minutes Subsequently the samples were stored in straws and subjected to freezing. After thawing, samples were evaluated following sperm parameters: sperm analysis of the kinetics through a computerized system (CASA), the sperm membrane integrity and mitochondrial potential by epifluorescence microscopy. For statistical analysis it was used ANOVA and Tukey's test where appropriate, with significance level P < 0.05. For the kinetic analysis of sperm was found that the values of linear velocity by G1 (82.3μm/s) were lower as compared to G2 (90.5μm/s) and C (89.7μm/s) and for curvilinear velocity, group C (206.6μm/s) showed values higher than G1 (183.0μm/s) and G2 (194.2μm/s) similar to both. For plasma membrane integrity G2 (34.7%) had significantly lower than C (39.4%) and G1 (41.6%), however mitochondrial function values were higher in group C (62, 6%) compared to G1 (47.5%); and, G2 (51.1%) was similar to both. Based on the results obtained can be conclude that 3 protocols adopted for cooling the freezing of semen sexed cattle showed few variation. Thus further studies are needed to improve the procedure of cryopreservation of bovine sexed semen. |
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