Near infrared spectroscopy determination of sucrose, glucose and fructose in sweet sorghum juice.

Sweet sorghumis a very robust cropwhich has the potential to be used in ethanol production due to its high fermentable sugar content present in its stem juice, very similar to sugarcane. Therefore, for breeding purposes it is relevant to analyze sugar composition in the juice to characterize sweet s...

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Bibliographic Details
Authors: SIMEONE, M. L. F., PARRELLA, R. A. C., SCHAFFERT, R. E., DAMASCENO, C. M. B., LEAL, M. C. B., PASQUINI, C.
Format: article
Status:Published version
Publication Date:2017
Country:Brasil
Institution:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repository:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Language:English
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/1076087
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076087
Access Level:Open access
Keyword:Calibração multivariada
Bioenergy crop
Bionergia
Biocombustível
Sorgo açucareiro
Biofuels
Energy crops
Description
Summary:Sweet sorghumis a very robust cropwhich has the potential to be used in ethanol production due to its high fermentable sugar content present in its stem juice, very similar to sugarcane. Therefore, for breeding purposes it is relevant to analyze sugar composition in the juice to characterize sweet sorghum genotypes and their period of industrial utilization within different environments for maximum ethanol yield. In this work we developed a rapid, lowcost and efficient method to determine the profile of sugars (sucrose, glucose and fructose) in sorghum juice by near infrared spectroscopy and partial least square regression, and validation of the method was performed according to the high-performance liquid chromatography method. Developed models provided root mean square error of prediction of 4, 1 and 0.6 mg·mL−1 and ratio performance deviations of 8, 5 and 5 for sucrose, glucose and fructose, respectively. Relative standard deviations of three sweet sorghumjuice sampleswere reportedwith content variation (low, mediumand high) 0.2, 0.3, 0.8% for sucrose; 1, 2, 2% for glucose; 1, 2, 3% for fructose. Sugar profile is an asset for crop breeders to take decisions for the development of more productive cultivars and higher sugar content.