Rapid method for simultaneous determination of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid isomers in milk by gc-fid
Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is...
| Autores: | , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2023 |
| País: | Brasil |
| Recursos: | Universidade Federal de Minas Gerais (UFMG) |
| Repositório: | Repositório Institucional da UFMG |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/78513 |
| Acesso em linha: | https://doi.org/10.21577/0100-4042.20230026 http://hdl.handle.net/1843/78513 https://orcid.org/0000-0003-3469-0284 https://orcid.org/0000-0001-5278-9865 https://orcid.org/0000-0002-8073-4236 https://orcid.org/0000-0002-1970-5882 https://orcid.org/0000-0001-6026-9608 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Fast GC CLA SLB-IL111 Fatty acids Milk Leite Nanopartículas Analise cromatografica |
| Resumo: | Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the baforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a fame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency. |
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