Reactivation of latent HIV-1 in vitro using an ethanolic extract from Euphorbia umbellata (Euphorbiaceae) latex
Euphorbia umbellata (E. umbellata) belongs to Euphorbiaceae family, popularly known as Janauba, and its latex contains a combination of phorbol esters with biological activities described to different cellular protein kinase C (PKC) isoforms. Here, we identified deoxi-phorbol esters present in E. um...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/56423 |
| Acceso en línea: | https://doi.org/10.1371/journal.pone.0207664 http://hdl.handle.net/1843/56423 http://orcid.org/0000-0002-9639-7940 https://orcid.org/0000-0001-5180-3717 https://orcid.org/0000-0001-9570-8244 https://orcid.org/0000-0001-5919-5242 https://orcid.org/0000-0002-9378-0418 https://orcid.org/0000-0001-6235-8807 https://orcid.org/0000-0002-2951-3964 https://orcid.org/0000-0001-8108-2500 https://orcid.org/0000-0002-0478-2332 |
| Access Level: | acceso abierto |
| Palabra clave: | Euphorbia umbellata HIV infection Latex extract Euforbiacea Infecções por HIV Latex |
| Sumario: | Euphorbia umbellata (E. umbellata) belongs to Euphorbiaceae family, popularly known as Janauba, and its latex contains a combination of phorbol esters with biological activities described to different cellular protein kinase C (PKC) isoforms. Here, we identified deoxi-phorbol esters present in E. umbellata latex alcoholic extract that are able to increase HIV transcription and reactivate virus from latency models. This activity is probably mediated by NF-kB activation followed by nuclear translocation and binding to the HIV LTR promoter. In addition, E. umbellata latex extract induced the production of pro inflammatory cytokines in vitro in human PBMC cultures. This latex extract also activates latent virus in human PBMCs isolated from HIV positive patients as well as latent SIV in non-human primate primary CD4+ T lymphocytes. Together, these results indicate that the phorbol esters present in E. umbellata latex are promising candidate compounds for future clinical trials for shock and kill therapies to promote HIV cure and eradication. |
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