Immunization of Balb/c mice with modified auto-antigens for induction of autoimmune sialoadenitis
Sjögren's syndrome is an autoimmune disease characterized by sialoadenitis and elevated titers of autoantibodies. To assess whether it is possible to induce inflammatory changes in salivary gland tissues, a series of immunizations in Balb/c mice have been undertaken, using salivary gland extrac...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/70065 |
| Acceso en línea: | http://dx.doi.org/10.1590/S0103-64402007000100009 http://hdl.handle.net/11449/70065 |
| Access Level: | acceso abierto |
| Palabra clave: | Autoimmune disease Carbonic anhydrase Salivary gland Sialoadenitis autoantigen mitogenic agent periodate periodic acid animal Bagg albino mouse cattle female hybridoma immunology mouse pathology salivary gland sialoadenitis Sjoegren syndrome vaccination Animals Autoantigens Cattle Female Hybridomas Mice Mice, Inbred BALB C Mitogens Periodic Acid Salivary Glands Sialadenitis Sjogren's Syndrome Vaccination |
| Sumario: | Sjögren's syndrome is an autoimmune disease characterized by sialoadenitis and elevated titers of autoantibodies. To assess whether it is possible to induce inflammatory changes in salivary gland tissues, a series of immunizations in Balb/c mice have been undertaken, using salivary gland extract, modified or not, added to several adjuvants. Mice's humoral immune response to salivary gland antigens was monitored by ELISA. Inflammatory cells infiltrating gland tissue were seen 3 months after immunization with salivary gland extract modified with pepsin (AgGp) and metaperiodate (AgGMp). Although pathological progression was not observed, the histopathological picture was similar to the initial phase of Sjögren's syndrome. In addition, a monoclonal antibody reactive with 3 gland polypeptides and anhydrase carbonic II was rescued among B cells from immunized mice. Thus, immunizations with modified autoantigens were able to initiate pathological damage to glandular tissue and stimulate the proliferation of auto-reactive B cells. |
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