14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity

Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic ma...

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Detalles Bibliográficos
Autores: Rivero, Guadalupe, Aldana, Ana Agustina, Frontini López, Yesica Romina, Liverani, L., Boccacini, A. R., Bustos, Diego Martin, Abraham, Gustavo Abel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/118907
Acceso en línea:http://hdl.handle.net/11336/118907
Access Level:acceso abierto
Palabra clave:PROTEIN INMOBILIZATION
ELECTROSPUN MEMBRANES
COMPOSITES
SCAFFOLDS
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
id AR_f0386ef73b5f57e2ce2d098fcd3e224e
oai_identifier_str oai:ri.conicet.gov.ar:11336/118907
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
title 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
spellingShingle 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
Rivero, Guadalupe
PROTEIN INMOBILIZATION
ELECTROSPUN MEMBRANES
COMPOSITES
SCAFFOLDS
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
title_short 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
title_full 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
title_fullStr 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
title_full_unstemmed 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
title_sort 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity
dc.creator.none.fl_str_mv Rivero, Guadalupe
Aldana, Ana Agustina
Frontini López, Yesica Romina
Liverani, L.
Boccacini, A. R.
Bustos, Diego Martin
Abraham, Gustavo Abel
author Rivero, Guadalupe
author_facet Rivero, Guadalupe
Aldana, Ana Agustina
Frontini López, Yesica Romina
Liverani, L.
Boccacini, A. R.
Bustos, Diego Martin
Abraham, Gustavo Abel
author_role author
author2 Aldana, Ana Agustina
Frontini López, Yesica Romina
Liverani, L.
Boccacini, A. R.
Bustos, Diego Martin
Abraham, Gustavo Abel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PROTEIN INMOBILIZATION
ELECTROSPUN MEMBRANES
COMPOSITES
SCAFFOLDS
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
topic PROTEIN INMOBILIZATION
ELECTROSPUN MEMBRANES
COMPOSITES
SCAFFOLDS
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
description Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds,and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118907
Rivero, Guadalupe; Aldana, Ana Agustina; Frontini López, Yesica Romina; Liverani, L.; Boccacini, A. R.; et al.; 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity; Springer; Journal of Materials Science: Materials In Medicine; 30; 9; 8-2019
0957-4530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118907
identifier_str_mv Rivero, Guadalupe; Aldana, Ana Agustina; Frontini López, Yesica Romina; Liverani, L.; Boccacini, A. R.; et al.; 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity; Springer; Journal of Materials Science: Materials In Medicine; 30; 9; 8-2019
0957-4530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10856-019-6302-2
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10856-019-6302-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196111388278784
spelling 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicityRivero, GuadalupeAldana, Ana AgustinaFrontini López, Yesica RominaLiverani, L.Boccacini, A. R.Bustos, Diego MartinAbraham, Gustavo AbelPROTEIN INMOBILIZATIONELECTROSPUN MEMBRANESCOMPOSITESSCAFFOLDShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds,and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL.Fil: Rivero, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Aldana, Ana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Frontini López, Yesica Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Liverani, L.. Universitat Erlangen-Nuremberg; AlemaniaFil: Boccacini, A. R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaSpringer2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118907Rivero, Guadalupe; Aldana, Ana Agustina; Frontini López, Yesica Romina; Liverani, L.; Boccacini, A. R.; et al.; 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity; Springer; Journal of Materials Science: Materials In Medicine; 30; 9; 8-20190957-4530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10856-019-6302-2info:eu-repo/semantics/altIdentifier/doi/10.1007/s10856-019-6302-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:15:08Zoai:ri.conicet.gov.ar:11336/118907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:15:08.412CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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