Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803

Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have p...

Descripción completa

Detalles Bibliográficos
Autores: Aguillera, Anabella, Steelheart Molina, María Charlotte, Alegre, Matías Leonel, Berdun, Federico, Salerno, Graciela Lidia, Bartoli, Carlos Guillermo, Pagnussat, Gabriela Carolina, Martin, María Victoria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/131844
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131844
Access Level:acceso abierto
Palabra clave:Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
id AR_edb0c81e5d0e6c9cc5c247bcd2a48d64
oai_identifier_str oai:sedici.unlp.edu.ar:10915/131844
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803Aguillera, AnabellaSteelheart Molina, María CharlotteAlegre, Matías LeonelBerdun, FedericoSalerno, Graciela LidiaBartoli, Carlos GuillermoPagnussat, Gabriela CarolinaMartin, María VictoriaCiencias NaturalesBiologíaCyanobacteriaAntioxidantsGlutathioneAscorbic acidOxidative stressAscorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.Instituto de Fisiología Vegetal2020-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/131844enginfo:eu-repo/semantics/altIdentifier/issn/2331-8325info:eu-repo/semantics/altIdentifier/doi/10.21769/bioprotoc.3800info:eu-repo/semantics/altIdentifier/pmid/33659454info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:10:08Zoai:sedici.unlp.edu.ar:10915/131844Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:10:09.199SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
spellingShingle Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
Aguillera, Anabella
Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
title_short Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_full Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_fullStr Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_full_unstemmed Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_sort Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
dc.creator.none.fl_str_mv Aguillera, Anabella
Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
author Aguillera, Anabella
author_facet Aguillera, Anabella
Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
author_role author
author2 Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
topic Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
description Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131844
url http://sedici.unlp.edu.ar/handle/10915/131844
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2331-8325
info:eu-repo/semantics/altIdentifier/doi/10.21769/bioprotoc.3800
info:eu-repo/semantics/altIdentifier/pmid/33659454
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1799194422660825088
score 15,81155

Similares en LA Referencia