Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803

Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have p...

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Detalles Bibliográficos
Autores: Aguillera, Anabella, Steelheart Molina, María Charlotte, Alegre, Matías Leonel, Berdun, Federico, Salerno, Graciela Lidia, Bartoli, Carlos Guillermo, Pagnussat, Gabriela Carolina, Martin, María Victoria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/131844
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131844
Access Level:acceso abierto
Palabra clave:Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
Descripción
Sumario:Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.

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