A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/94353 |
| Acceso en línea: | http://hdl.handle.net/11336/94353 |
| Access Level: | acceso abierto |
| Palabra clave: | EUDRAGIT L 100 EUDRAGIT S 100 PEROXIDASE POLYELECTROLYTES PURIFICATION https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| Sumario: | The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit® concentration to precipitate the enzyme was very low: about 2 × 10 -3% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times. |
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