A liposome-actuated enzyme system and its capability as a self-biomineralized silica nanoreactor
Controlling the functions that arise in biomolecular nanostructures is a major challenge in biotechnology developments. Liposomes have been used to encapsulate a wide variety of enzymes, but the emergent properties of systems in which enzymatic activity is controlled by surrounding liposomes have no...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/83227 |
| Acceso en línea: | http://hdl.handle.net/11336/83227 |
| Access Level: | acceso abierto |
| Palabra clave: | Liposome Nanocapsule Enzymes Biomineralization https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
| Sumario: | Controlling the functions that arise in biomolecular nanostructures is a major challenge in biotechnology developments. Liposomes have been used to encapsulate a wide variety of enzymes, but the emergent properties of systems in which enzymatic activity is controlled by surrounding liposomes have not been explored. Here, we report an assembly to actuate the reactivity of ureases through the change of the liposome permeability by means of temperature control. Using this approach, externally controlled enzyme inhibition and activation are successfully demonstrated. Furthermore, deposited on a sacrificial mesoporous thin film, this biosystem controllably rebuilds its surface to generate a 3D silica shell, which provides an additional demonstration to the proposed principle. |
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